Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial CellsJeffrey M. Finlon 1, Matthew A. Burchill 1, Beth A. Jirón Tamburini 1,2
1Division of Gastroenterology and Hepatology, Department of Medicine, University of Colorado Anschutz Medical Campus, School of Medicine, 2Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus
The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.
