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JoVE Journal

Immunology and Infection

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Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

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08:07 min

January 7th, 2019

January 7th, 2019

10,426 Views

0:04

Title

0:52

Liver Single-cell Suspension Preparation

3:36

Flow Cytometric Analysis

5:49

Results: Representative Murine Liver Lymphatic Endothelial Cell (LEC) Characterization

7:22

Conclusion

필기록

This method can help answer key questions in lymphatic and liver fields, such as how lymphatic endothelial cells, or LECs, respond to specific stimuli and how that contributes to disease pathogenesis. The main advantage of this technique is that w

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The goal of this protocol is to identify lymphatic endothelial cell populations within the liver using described markers. We utilize collagenase IV and DNase and a gentle mincing of tissue, combined with flow cytometry, to identify a distinct population of lymphatic endothelial cells.

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