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USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology

2 ARTICLES PUBLISHED IN JoVE

Environment

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Neil D. Sanscrainte¹, Christy M. Waits^2,3, Christopher J. Geden¹, Alden S. Estep^1,2, James J. Becnel¹

¹USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology, ²CMAVE Detachment, Navy Entomology Center of Excellence, ³Lovelace Respiratory Research Institute

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.

Immunology and Infection

Propagation of the Microsporidian Parasite Edhazardia aedis in Aedes aegypti Mosquitoes

Anthony Grigsby^*1, Brendan J. Kelly^*1, Neil D. Sanscrainte², James J. Becnel², Sarah M. Short¹

¹Department of Entomology, The Ohio State University, ²USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology

A protocol to culture the microsporidian parasite Edhazardia aedis. The parasite is passaged from one generation of Aedes aegypti mosquitoes to the next via horizontal transfer at the larval stage followed by vertical transmission at the adult stage. Live sporoplasms survive long-term in infected eggs.