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University of New Mexico Health Science Center

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Julie G. In^*1, Jennifer Foulke-Abel^*2, Elizabeth Clarke¹, Olga Kovbasnjuk¹

¹Department of Internal Medicine, University of New Mexico Health Science Center, ²Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.