Transcription Start Site Mapping Using Super-low Input Carrier-CAGENevena Cvetesic 1,2, Elena Pahita 1,2, Boris Lenhard 1,2,3
1Institute of Clinical Sciences, Faculty of Medicine, Imperial College London, 2MRC London Institute of Medical Sciences, 3Sars International Centre for Marine Molecular Biology, University of Bergen
Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.
