Inducing Meningococcal Meningitis Serogroup C in Mice via Intracisternal DeliveryChiara Pagliuca 1, Elena Scaglione 1, Francesca Carraturo 1, Giuseppe Mantova 1, Maria Michela Marino 1, Maria Virginia Pishbin 1, Caterina Pagliarulo 2, Roberta Colicchio 1, Paola Salvatore 1,3
1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology
Here, we describe a method to induce meningococcal meningitis through an intracisternal route of infection in adult mice. We present a step by step protocol of meningococcal infection from the preparation of inoculum to the intracisternal infection; then record the animal survival and evaluate the bacterial loads in murine tissues.
