Determination of Immune Cell Identity and Purity Using Epigenetic-Based Quantitative PCRSuman K. Pradhan 1, Jerry Guzman 1, Carl Dargitz 1, Stephanie Switalski 2, Mark Landon 1, Sven Olek 3, Bjoern Samans 3, Ulrich Hoffmueler 3, Uma Lakshmipathy 1
1Cell Biology, Life Sciences Solutions, Thermo Fisher Scientific, 2California Polytechnic State University (Cal Poly), 3Epiontis GmbH, Precision for Medicine
Here we describe a robust method of determining immune cell identity and purity through epigenetic signatures detected using quantitative PCR (qPCR). DNA demethylation at a specific locus serves as a unique identifier for a particular cell type and allows for identification of CD8+, regulatory, or Th17 T cells.
