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Universidade de Santiago de Compostela

2 ARTICLES PUBLISHED IN JoVE

Chemistry

Synthesis of Functionalized Magnetic Nanoparticles, Their Conjugation with the Siderophore Feroxamine and its Evaluation for Bacteria Detection

Diana Martínez-Matamoros¹, Socorro Castro-García¹, Gabriela Ojeda Romano², Miguel Balado³, Jaime Rodríguez¹, Manuel L. Lemos³, Carlos Jiménez¹

¹Centro de Investigacións Científicas Avanzadas (CICA), Departamento de Química, Facultade de Ciencias, Universidade da Coruña, ²Centro de Investigacións Científicas Avanzadas (CICA), Unidad de Comunicación y Divulgación, Universidade da Coruña, ³Department of Microbiology and Parasitology, Institute of Aquaculture, Universidade de Santiago de Compostela

This work describes protocols for the preparation of magnetic nanoparticles, its coating with SiO₂, followed by its amine functionalization with (3-aminopropyl)triethoxysilane (APTES) and its conjugation with deferoxamine using a succinyl moiety as a linker. A deep structural characterization description and a capture bacteria assay using Y. enterocolitica for all the intermediate nanoparticles and the final conjugate are also described in detail.

Chemistry

Microscopic Visualization of Porous Nanographenes Synthesized through a Combination of Solution and On-Surface Chemistry

Rafal Zuzak¹, Iago Pozo², Mads Engelund³, Manuel Vilas-Varela², José M. Alonso², Enrique Guitián², Dolores Pérez², Diego Peña², Szymon Godlewski¹

¹Centre for Nanometer-Scale Science and Advanced Materials, NANOSAM, Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, ²Centro de Investigación en Química Biolóxica e Materiais Moleculares (CiQUS), Departamento de Química Orgánica, Universidade de Santiago de Compostela, ³Espeem S.A.R.L.

A combination of solution and surface assisted synthesis opens new directions in the atomically precise synthesis of nanostructures. Scanning tunneling microscopy (STM) supplemented by non-contact atomic force microscopy (nc-AFM) enables detailed characterization of newly designed and generated carbon-based nano-objects.