Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live CellsGiulia Bertolin 1, Gilles Le Marchand 1, Marc Tramier 1
1Univ Rennes, CNRS, IGDR (Genetics and Development Institute of Rennes), UMR 6290, F-35000 Rennes, France
The activation of the multifunctional Ser/Thr kinase AURKA is hallmarked by its autophosphorylation on Thr288. Fluorescent probes relying on FRET can discriminate between its inactive and activated states. Here, we illustrate some strategies for probe engineering, together with a rapid FRET protocol to follow the kinase activation throughout mitosis.
