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Université Bordeaux

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Chemistry

Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies
Sofia Jaho 1, Niels Junius 1,3, Franck Borel 1, Yoann Sallaz-Damaz 1, Jean-Baptiste Salmon 2, Monika Budayova-Spano 1
1Université Grenoble Alpes, CEA, CNRS, IBS, 2CNRS, Solvay LOF, UMR 5258, Université Bordeaux, 3ELVESYS SAS

This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip.

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