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Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard

1 ARTICLES PUBLISHED IN JoVE

Cancer Research

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Maria Virginia Giolito^1,2, Léo Claret^1,2, Carla Frau¹, Michelina Plateroti^1,2

¹Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, ²UMR-S1113 - IRFAC INSERM, Université de Strasbourg

This protocol presents a novel, robust, and reproducible culture system to generate and grow three-dimensional spheroids from Caco2 colon adenocarcinoma cells. The results provide the first proof-of-concept for the appropriateness of this approach to study cancer stem cell biology, including the response to chemotherapy.