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University Hospital of Liège

2 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

Nasal Brushing Sampling and Processing Using Digital High Speed Ciliary Videomicroscopy – Adaptation for the COVID-19 Pandemic

Noemie Bricmont¹, Lionel Benchimol², Anne-Lise Poirrier², Christine Grignet³, Claire Seaton⁴, Mark A. Chilvers⁴, Marie-Christine Seghaye⁵, Renaud Louis^1,6, Philippe Lefebvre², Celine Kempeneers^1,7

¹Pneumology laboratory, I3 Group, GIGA Research Center, University of Liège, ²Department of ENT, University Hospital of Liège, ³Department for Occupational Safety and Health, Biosafety and Biosecurity Unit, University of Liège, ⁴Division of Respirology, Department of Pediatrics, University of British Columbia and British Columbia Children's Hospital, ⁵Division of Cardiology, Department of Pediatrics, University Hospital of Liège, ⁶Department of Pneumology, University Hospital of Liège, ⁷Division of Respirology, Department of Pediatrics, University Hospital of Liège

To guarantee a successful and high-quality ciliary functional analysis for PCD diagnosis, a precise and careful method for respiratory epithelium sampling and processing is essential. To continue providing PCD diagnostic service during the COVID-19 pandemic, the ciliary videomicroscopy protocol has been updated to include appropriate infection control measures.

Behavior

SECONDs Administration Guidelines: A Fast Tool to Assess Consciousness in Brain-injured Patients

Leandro R. D. Sanz^*1,2, Charlène Aubinet^*1,2, Helena Cassol^1,2, Olivier Bodart^1,2, Sarah Wannez^1,2, Estelle A. C. Bonin^1,2, Alice Barra^1,2, Nicolas Lejeune^1,2,3,4, Charlotte Martial^1,2, Camille Chatelle^1,2, Didier Ledoux^5,6, Steven Laureys^1,2, Aurore Thibaut^1,2, Olivia Gosseries^1,2

¹Coma Science Group, GIGA-Consciousness, GIGA research center, University of Liège, ²Centre du Cerveau, University Hospital of Liège, ³CHN William Lennox, Groupe Hospitalier Saint-Luc, ⁴Institute of NeuroScience, UCLouvain, ⁵Department of Intensive Care, University Hospital of Liège, ⁶Anesthesia & Intensive Care Laboratory, GIGA-Consciousness, GIGA research center, University of Liège

These guidelines can be used for the administration of the Simplified Evaluation of CONsciousness Disorders (SECONDs), a short behavioral tool developed to diagnose brain-injured patients in time-constrained settings. This scale examines command-following, communication, visual pursuit, fixation, pain localization, oriented movements and arousal.