NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation ModeHannes Berg *1, M. A. Wirtz Martin *1, A. Niesteruk 1,2, C. Richter 1, S. Sreeramulu 1, H. Schwalbe 1,2
1Institute for Organic Chemistry and Chemical Biology, Center for Biomolecular Magnetic Resonance (BMRZ), Johann Wolfgang Goethe-University Frankfurt, 2German Cancer Consortium (DKTK) and DKFZ
Fragment-based screening by NMR is a robust method to rapidly identify small molecule binders to biomacromolecules (DNA, RNA, or proteins). Protocols describing automation-based sample preparation, NMR experiments & acquisition conditions, and analysis workflows are presented. The technique allows for optimal exploitation of both 1H and 19F NMR-active nuclei for detection.
