Preparing Lamellae from Vitreous Biological Samples Using a Dual-Beam Scanning Electron Microscope for Cryo-Electron TomographyClaudine Bisson 1,2, Corey W. Hecksel 3,4, James B. Gilchrist 3, M. Alejandra Carbajal 1, Roland A. Fleck 1
1Centre for Ultrastructural Imaging, New Hunt’s House, Guy’s Campus, King’s College London, 2Department of Biological Science, Birkbeck College, University of London, 3Electron Bio-Imaging Centre, Diamond Light Source, Harwell Science and Innovation Campus, 4SLAC National Accelerator Laboratory, Stanford University
Using focused ion beam milling to produce vitreous on-grid lamellae from plunge frozen biological samples for cryo-electron tomography.
