Medium-Throughput Drug- and Radiotherapy Screening Assay using Patient-Derived OrganoidsMarrit Putker *1, Rosemary Millen *2, René Overmeer *3, Else Driehuis 2,4, Maurice M. J. M. Zandvliet 5, Hans Clevers 2, Sylvia F. Boj 3, Qi-Xiang Li 6
1Crown Bioscience Netherlands B.V., 2Oncode Institute, Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center Utrecht, 3Hubrecht Organoid Technology (HUB), 4Pathology dept, University Medical Center Utrecht, 5Department of Clinical Sciences - Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 6Crown Bioscience Inc.
We describe detailed protocols to use patient-derived organoids for medium-throughput therapy sensitivity screenings. Therapies tested include chemotherapy, radiotherapy, and chemo-radiotherapy. Adenosine triphosphate levels are used as a functional readout.
