Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual MicrodissectionVeronica Ibarra-Lopez 1, Sangeeta Jayakar 1, Yeqing Angela Yang 2, Ciara Martin 3, Zora Modrusan 2, Sandra Rost 1
1Department of Pathology, Genentech, 2Department of Microchemistry, Proteomics, Lipidomics & Next Generation Sequencing (MPL-NGS), Genentech, 3NanoString Technologies
Here, we describe a protocol for fine-tuning regions of interest (ROIs) for Spatial Omics technologies to better characterize the tumor microenvironment and identify specific cell populations. For proteomics assays, automated customized protocols can guide ROI selection, while transcriptomics assays can be fine-tuned utilizing ROIs as small as 50 µm.
