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University of Florida, Gainesville

3 ARTICLES PUBLISHED IN JoVE

Behavior

Use of the Operant Orofacial Pain Assessment Device (OPAD) to Measure Changes in Nociceptive Behavior

Ethan M. Anderson^1,2, Richard Mills³, Todd A. Nolan⁴, Alan C. Jenkins⁴, Golam Mustafa¹, Chris Lloyd³, Robert M. Caudle^1,2, John K. Neubert⁴

¹Department of Oral and Maxillofacial Surgery, University of Florida College of Dentistry, ²Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine , ³Stoelting Co., ⁴Department of Orthodontics, University of Florida

We present a user-friendly, high-throughput operant system for the evaluation of pain behaviors in awake, conscious rodents. The Orofacial Pain Assessment Device (OPAD) can assess pain through a reward/conflict paradigm thus providing a more humane way of testing. This protocol will yield more clinically relevant and translational data from rodents.

Immunology and Infection

Adapting Gastrointestinal Organoids for Pathogen Infection and Single Cell Sequencing under Biosafety Level 3 (BSL-3) Conditions

Megan L. Stanifer^1,2, Steeve Boulant^2,3,4

¹Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, ²Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, ³Department of Infectious Diseases, Virology, Heidelberg University Hospital, ⁴Research Group “Cellular Polarity and Viral Infection”, German Cancer Research Center (DKFZ)

This protocol describes how to infect human intestinal organoids from either their apical or basolateral side to characterize host/pathogen interactions at the single-cell level using single-cell RNA sequencing (scRNAseq) technology.

Behavior

Assessment of Nerve Injury-Induced Mechanical Hypersensitivity in Rats Using an Orofacial Operant Pain Assay

Basak Donertas-Ayaz¹, Ariana C. Brice-Tutt², Wendi L. Malphurs², Darrice Montgomery², Richard H. Mills³, John K. Neubert², Robert M. Caudle¹

¹Department of Oral and Maxillofacial Surgery, University of Florida, Gainesville, ²Department of Orthodontics, University of Florida, Gainesville, ³Stoelting Co. Wood Dale

This protocol describes the assessment of mechanical hypersensitivity in a rat model of neuropathic orofacial pain using an operant-based orofacial pain assessment device.