High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T CellsBruce Nguyen Tran 1, Eunkeu Oh 2, Kimihiro Susumu 2,3, Mason Wolak 2, Kirill Gorshkov 1
1National Center for Advancing Translational Sciences, 2Optical Sciences Division, Code 5600, Naval Research Laboratory, 3Jacobs Corporation
In this protocol, quantum dots conjugated to recombinant SARS-CoV-2 spike enable cell-based assays to monitor spike binding to hACE2 at the plasma membrane and subsequent endocytosis of the bound proteins into the cytoplasm.
