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Universidad Autónoma Metropolitana

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Computational Reconstruction of Pancreatic Islets as a Tool for Structural and Functional Analysis

Gerardo J. Félix-Martínez^1,2, Aurelio Nicolás-Mata², J. Rafael Godínez-Fernández²

¹Department of Electrical Engineering, Universidad Autónoma Metropolitana

In this protocol, the pancreatic islets are reconstructed and analyzed using computational algorithms implemented in a dedicated multiplatform application.

Biology

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

Aymé Oliva Cárdenas^1,2, Blanca C. Zamora-Rodríguez², Kevin A. Batalla-García², Alejandro Ávalos-Rodríguez³, Alejandra Contreras-Ramos⁴, Clara Ortega-Camarillo²

¹Doctorado en Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, ²Medical Research Unit in Biochemistry, Specialties Hospital, National Medical Center SXXI, Instituto Mexicano de Seguro Social, ³Department of Agricultural and Animal Production, Division of Biological and Health Science, Universidad Autónoma Metropolitana-Xochimilco, ⁴Molecular Biology Research Laboratory, Center for Congenital Malformations, Children Hospital of Mexico Federico Gomez (HIMFG)

This protocol describes a methodology for isolating and identifying adipose tissue-derived mesenchymal stem cells (MSCs) from Sprague Dawley rats.