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Université d'Orléans

2 ARTICLES PUBLISHED IN JoVE

JoVE Core

Postural Organization of Gait Initiation for Biomechanical Analysis Using Force Platform Recordings

Arnaud Simonet^1,2,3, Arnaud Delafontaine^2,3,4, Paul Fourcade^2,3, Eric Yiou^2,3

¹LADAPT Loiret, Centre de Soins de Suite et de Réadaptation, ²CIAMS, Université Paris-Saclay, ³CIAMS, Université d'Orléans, ⁴Université Libre de Bruxelles, Belgique

This paper describes the material and method developed to investigate the postural organization of gait initiation. The method is based on force platform recordings and on the direct principle of mechanics to compute center of gravity and center of pressure kinematics.

Biochemistry

Optimized Methods for the Surface Immobilization of Collagens and Collagen Binding Assays

Nadia Chaher¹, Giuseppe Digilio^*2, Sara Lacerda^*3, René M. Botnar^1,4,5,6, Alkystis Phinikaridou^1,4

¹School of Biomedical Engineering and Imaging Sciences, King’s College London, ²Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale, ³Centre de Biophysique Moléculaire, CNRS UPR 4301, Université d'Orléans, ⁴BHF Centre of Research Excellence, Cardiovascular Division, King’s College London, ⁵Escuela de Ingeniería, Pontificia Universidad Católica de Chile, ⁶Instituto de Ingeniería Biológica y Médica, Pontificia Universidad Católica de Chile

This work presents an optimized protocol to reproducibly immobilize and quantify type I and III collagen onto microplates, followed by an improved in vitro binding assay protocol to study collagen-compound interactions using a time-resolved fluorescence method. The subsequent step-by-step data analysis and data interpretation are provided.