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Porto Comprehensive Cancer Center (P.CCC)

1 ARTICLES PUBLISHED IN JoVE

JoVE Core

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Laura Mosteo¹, Joana Reis¹, Lídia Rocha¹, Marta Lopes¹, Delfim Duarte^1,2,3,4

¹Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, ²Department of Onco-Hematology, Instituto Português de Oncologia (IPO)-Porto, ³Unit of Biochemistry, Department of Biomedicine, Faculdade de Medicina da Universidade do Porto, ⁴Porto Comprehensive Cancer Center (P.CCC)

Here, we describe a simple protocol for the isolation and staining of murine bone marrow cells to phenotype hemopoietic stem and progenitor cells along with the supporting niche endothelial and mesenchymal stem cells. A method to enrich cells located in endosteal and central bone marrow areas is also included.