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Amsterdam University Medical Center

2 ARTICLES PUBLISHED IN JoVE

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Bioengineering

Generation, High-Throughput Screening, and Biobanking of Human-Induced Pluripotent Stem Cell-Derived Cardiac Spheroids
Renee G. C. Maas 1, Tess Beekink *1, Nino Chirico *1, Christian J. B. Snijders Blok 1, Inge Dokter 1, Vasco Sampaio-Pinto 1, Alain van Mil 1, Pieter A. Doevendans 1, Jan W. Buikema 2, Joost P. G. Sluijter *1, Francesca Stillitano *1
1Utrecht Regenerative Medicine Center, Circulatory Health Laboratory, University Utrecht, Department of Cardiology, University Medical Center Utrecht, 2Amsterdam Cardiovascular Sciences, Department of Physiology, Amsterdam University Medical Center

Presented here is a set of protocols for the generation and cryopreservation of cardiac spheroids (CSs) from human-induced pluripotent stem cell-derived cardiomyocytes cultured in a high-throughput, multidimensional format. This three-dimensional model functions as a robust platform for disease modeling, high-throughput screenings, and maintains its functionality after cryopreservation.

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Developmental Biology

Human Ovarian Surface Epithelium Organoids as a Platform to Study Tissue Regeneration
Julieta S. Del Valle 1,2, Azra Husetic *1, Dina Diek *1, Laurens F. Rutgers *1, Joyce D. Asseler 3,4,5, Jeroen Metzemaekers 6, Norah M. van Mello 3,4,5, Susana M. Chuva de Sousa Lopes 1,2,7
1Department of Anatomy and Embryology, Leiden University Medical Center, 2The Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW), Leiden University Medical Center, 3Department of Obstetrics and Gynaecology, Amsterdam University Medical Center, 4Centre of Expertise on Gender Dysphoria, Amsterdam UMC, 5Amsterdam Reproduction and Development Research Institute, 6Department of Gynaecology, Leiden University Medical Center, 7Ghent-Fertility and Stem Cell Team (G-FAST), Department of Reproductive Medicine, Ghent University Hospital

This protocol describes establishing three-dimensional (3D) tissue organoids from primary human ovarian surface epithelium (hOSE) cells. The protocol includes isolation of hOSE from freshly collected ovaries, cellular expansion of the hOSE, cryopreservation-thawing procedures, and organoid derivation. Immunofluorescence, quantitative analysis, and showcasing utility as a screening platform are included.

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