Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic NicheThomas Kerloch 1, Tjaša Lepko 2, Kirill Shkura 2, François Guillemot 1, Sébastien Gillotin 2
1The Francis Crick Institute, 2MSD R&D Innovation Centre
Presented here is a method to sequence single nuclei isolated from the mouse dentate gyrus that excludes most neurons through fluorescence-activated nuclei (FAN)-sorting. This approach generates high-quality expression profiles and facilitates the study of most other cell types represented in the niche, including scarce populations such as neural stem cells.
