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Ludwig-Maximilians-University Munich (LMU)

5 ARTICLES PUBLISHED IN JoVE

Medicine

Isolation and Culture of Resident Cardiac Macrophages from the Murine Sinoatrial and Atrioventricular Node

Ruibing Xia^1,2,3, Simone Loy¹, Stefan Kääb^1,3, Anna Titova¹, Christian Schulz^1,2,3, Steffen Massberg^1,2,3, Sebastian Clauss^1,2,3

¹University Hospital Munich, Department of Medicine I, Ludwig-Maximilian-Unversity Munich (LMU), ²Insitute of Surgical Research at the Walter Brendel Center of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians-University (LMU), ³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA)

The protocol presented here provides a step-by-step approach for the isolation of cardiac resident macrophages from the sinoatrial node (SAN) and atrioventricular node (AVN) region of mouse hearts.

Medicine

Analyzing Long-Term Electrocardiography Recordings to Detect Arrhythmias in Mice

Philipp Tomsits^1,2,3, Kavi Raj Chataut^1,2, Aparna Sharma Chivukula^1,2, Li Mo^1,2, Ruibing Xia^1,2, Dominik Schüttler^1,2,3, Sebastian Clauss^1,2,3

¹Department of Medicine I, University Hospital Munich, Campus Großhadern, Ludwig-Maximilians University Munich (LMU), ²DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), ³Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians University Munich (LMU)

Here we present a step-by-step protocol for a semiautomated approach to analyze murine long-term electrocardiography (ECG) data for basic ECG parameters and common arrhythmias. Data are obtained by implantable telemetry transmitters in living and awake mice and analyzed using Ponemah and its analysis modules.

Medicine

Real-Time Electrocardiogram Monitoring During Treadmill Training in Mice

Philipp Tomsits^*1,2,4, Aparna Sharma Chivukula^*1,2, Kavi Raj Chataut^*1,2, Agus Simahendra³, Ludwig T. Weckbach^1,2,3, Stefan Brunner¹, Sebastian Clauss^1,2,4

¹Department of Medicine I, University Hospital Munich, Ludwig-Maximilians University Munich (LMU), ²DZHK (German Centre for Cardiovascular Research), Partner Site Munich, Munich Heart Alliance (MHA), ³Institute of Cardiovascular Physiology and Pathophysiology, Biomedical Center, Ludwig-Maximilians-University, ⁴Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital Munich, Ludwig-Maximilians University Munich (LMU)

Electrocardiogram (ECG) is the key variable to understanding cardiac electrophysiology. Physical exercise has beneficial effects but may also be harmful in the context of cardiovascular diseases. This manuscript provides a method of recording real-time ECG during exercise, which can serve to investigate its effects on cardiac electrophysiology in mice.

An Innovative Toolkit to Investigate the Complex Mechanisms of Cardiac Arrhythmias

Sebastian Clauss^1,2,3,4

¹Department of Medicine I, University Hospital Munich (LMU), Ludwig-Maximilians-University Munich (LMU), ²Institute of Surgical Research at the Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, ³DZHK (German Centre for Cardiovascular Research) Partner Site Munich, Munich Heart Alliance (MHA), ⁴Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich

An Innovative Toolkit to Investigate the Complex Mechanisms of Cardiac Arrhythmias

Medicine

Microdissection and Immunofluorescence Staining of Myocardial Sleeves in Murine Pulmonary Veins

Hannes E. Villgrater^*1,2,3, Ruibing Xia^*1,2,3, Aparna Sharma Chivukula^1,2,3, Philipp Tomsits^*1,2,3, Sebastian Clauss^*1,2,3,4

¹University Hospital Munich, Department of Medicine I, Ludwig-Maximilians-University Munich (LMU), ²Walter Brendel Center of Experimental Medicine (WBex), LMU Munich, ³German Center for Cardiovascular Research (DZHK), Partner Site Munich, Munich Heart Alliance (MHA), ⁴Interfaculty Center for Endocrine and Cardiovascular Disease Network Modelling and Clinical Transfer (ICONLMU), LMU Munich

This protocol demonstrates microscopy-guided isolation and immunofluorescence staining of murine pulmonary veins. We prepare tissue samples containing the left atrium, pulmonary veins, and the corresponding lungs and stain them for cardiac Troponin T and Connexin 43.