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University of Dayton

2 ARTICLES PUBLISHED IN JoVE

Biology

A System for Culturing Iris Pigment Epithelial Cells to Study Lens Regeneration in Newt

Rital B. Bhavsar^*1, Kenta Nakamura^*1, Panagiotis A. Tsonis^1,2

¹Department of Biology, University of Dayton, ²Center for Tissue Regeneration and Engineering, University of Dayton

In newt, the lens regenerates always from the dorsal iris by transdifferentiation of the iris pigmented epithelial cells (IPEs). Here we describe a procedure to culture dorsal and ventral newt IPE cells and their implantation to the newt eye. The implanted cells are then studied by tissue sectioning and immunohistochemistry.

Biology

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

William A. Rogers¹, Thomas M. Williams²

¹Department of Biology, University of Dayton, ²Department of Biology, Center for Tissue Regeneration and Engineering at Dayton, University of Dayton

Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.