biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

large animal models

This collection features research methods using porcine, bovine, equine, and leporine (rabbit) models at their embryonic, neonatal, and adult stages. These techniques include ex-vivo and in-vivo studies, organ harvesting and preservation methods, cell isolation and culturing methodologies, microbial culturing, biochemical and biophysical assays, diagnostic tests, and various surgical procedures.

Large Animal Models

drosophila melanogaster (fruit fly)

This collection features research methods using the model organism Drosophila melanogaster at each stage of its life cycle to explore a wide range of physiological and behavioral questions.

Drosophila melanogaster (fruit fly)

caenorhabditis elegans (worm)

This collection features research techniques for the metazoan Caenorhabditis elegans. This nematode worm is a powerful model system due to its transparent body, defined developmental plan, robust genetic tools, and neuro-behavioral paradigms.

Caenorhabditis elegans (worm)

danio rerio (zebrafish)

This collection features research methods using the model organism Danio rerio in its embryo, larva, and adult stages to explore physiological and behavioral questions and create disease models for screening various chemicals.

Danio rerio (zebrafish)

rodent models

This collection features research methods using rodent models in embryonic, neonatal, and adult stages. The techniques include in vitro and in vivo organ perfusion, tissue and organ isolation, microbial culturing, biochemical studies, organ transplant methodologies, behavioral tests, and surgical procedures.

Rodent Models

basic methods

Basic Methods

microscopy

Microscopy

behavior

Behavior

anatomy and physiology

Anatomy and Physiology

cell biology

Cell Biology

genetics

Genetics

nematode synchronization: a method to obtain populations of worms in identical stages of development

nematode-synchronization-method-to-obtain-populations-worms-identical

Nematode Synchronization: A Method to Obtain Populations of Worms in Identical Stages of Development

Source: Sarasija, S. and Norman, K. R. Measurement of Oxygen Consumption Rates in Intact&#160;Caenorhabditis elegans.&#160;J. Vis. Exp. (2019).
In this ...

single worm pcr: a method to extract and amplify genomic dna

single-worm-pcr-a-method-to-extract-and-amplify-genomic-dna

Single Worm PCR: A Method to Extract and Amplify Genomic DNA

Source: Prior, H., et al. A Rapid and Facile Pipeline for Generating Genomic Point Mutants in&#160;C. elegans&#160;Using CRISPR/Cas9 ...

lifespan analysis: measuring c. elegans longevity

lifespan-analysis-measuring-c-elegans-longevity

Lifespan Analysis: Measuring C. elegans Longevity

Source: Cornwell, A. B., et al. The Replica Set Method: A High-throughput Approach to Quantitatively Measure&#160;Caenorhabditis ...

egg laying assay: a method to quantify the egg-laying behavior of c. elegans

egg-laying-assay-method-to-quantify-egg-laying-behavior-c

Egg Laying Assay: A Method to Quantify the Egg-Laying Behavior of C. elegans

Source: Lee, S. Y. and Kang, K. Measuring the Effect of Chemicals on the Growth and Reproduction of&#160;Caenorhabditis elegans.&#160;J. Vis. Exp. 
...

gonad microinjection: a method of compound delivery directly into the germline of c. elegans

gonad-microinjection-method-compound-delivery-directly-into-germline

Gonad Microinjection: A Method of Compound Delivery Directly into the Germline of C. elegans

Source: Farboud, B. et al, Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms.&#160;J. Vis. Exp. (2018).
This video ...

freeze-cracking of nematodes: a method to expose interior worm tissues for staining

freeze-cracking-nematodes-method-to-expose-interior-worm-tissues-for

Freeze-Cracking of Nematodes: A Method to Expose Interior Worm Tissues for Staining

Source: Zhang, N., et al. The C. elegans Intestine As a Model for Intercellular Lumen Morphogenesis and In Vivo Polarized Membrane Biogenesis at the ...

nematode slide preparation: a method to mount animals on an agar pad

nematode-slide-preparation-a-method-to-mount-animals-on-an-agar-pad

Nematode Slide Preparation: A Method to Mount Animals on an Agar Pad

Source: Teoh, J. S., et al. Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans. J. Vis. Exp. ...

calcium imaging: a method to visualize neural activity in live c. elegans

calcium-imaging-method-to-visualize-neural-activity-live-c

Calcium Imaging: A Method to Visualize Neural Activity in Live C. elegans

Source: Turek, M. et al. Agarose Microchambers for Long-term Calcium Imaging of&#160;Caenorhabditis elegans.&#160;J. Vis. Exp.&#160;(2015).
This video ...

swimming-induced paralysis (swip) assay: a method to quantify dopamine-mediated locomotion in c. elegans

swimming-induced-paralysis-swip-assay-method-to-quantify-dopamine

Swimming-Induced Paralysis (SWIP) Assay: A Method to Quantify Dopamine-Mediated Locomotion in C. elegans

Source: Kudumala, S., et al. Swimming Induced Paralysis to Assess Dopamine Signaling in&#160;Caenorhabditis elegans.&#160;J. Vis. Exp.&#160;(2019).
Here, ...

touch habituation assay: a method to study non-associative learning in c. elegans

touch-habituation-assay-method-to-study-non-associative-learning-c

Touch Habituation Assay: A Method to Study Non-Associative Learning in C. elegans

Source: Mersha, M. D., et al. Long-term Behavioral and Reproductive Consequences of Embryonic Exposure to Low-dose Toxicants. J. Vis. Exp. (2018).
This ...

c. elegans chemotaxis assay: a method to test chemosensation in worms

c-elegans-chemotaxis-assay-a-method-to-test-chemosensation-in-worms

C. elegans Chemotaxis Assay: A Method to Test Chemosensation in Worms

Source: Campbell, J. C., et al. A Caenorhabditis elegans Nutritional-Status Based Copper Aversion Assay. J. Vis. Exp. (2017).
This video introduces ...

electrotaxis assay: a method to observe locomotion in c. elegans

electrotaxis-assay-a-method-to-observe-locomotion-in-c-elegans

Electrotaxis Assay: A Method to Observe Locomotion in C. elegans

Source: Tong, J,&#160;et al. Microfluidic-based Electrotaxis for On-demand Quantitative Analysis of&#160;Caenorhabditis elegans&#39; Locomotion.&#160;J. ...

c. elegans movement tracking: a method to assess locomotion in worms

c-elegans-movement-tracking-a-method-to-assess-locomotion-in-worms

C. elegans Movement Tracking: A Method to Assess Locomotion in Worms

Source: Davies, A. G. et al. An Assay for Measuring the Effects of Ethanol on the Locomotion Speed of&#160;Caenorhabditis elegans.&#160;J. Vis. ...

c. elegans gonad extrusion: a rapid dissection technique

c-elegans-gonad-extrusion-a-rapid-dissection-technique

C. elegans Gonad Extrusion: A Rapid Dissection Technique

Source: Gervaise, A. L. and Arur, S. Spatial and Temporal Analysis of Active ERK in the C. elegans Germline. J. Vis. Exp. (2016).
This video describes a ...

c. elegans survival assay: a short-term toxicity test

c-elegans-survival-assay-a-short-term-toxicity-test

C. elegans Survival Assay: A Short-Term Toxicity Test

Source: Possik, E. and Pause, A. Measuring Oxidative Stress Resistance of&#160;Caenorhabditis elegans&#160;in 96-well Microtiter Plates.&#160;J. Vis. Exp. ...

c. elegans muscle area measurements: a standardized method to quantify striated muscle morphology

c-elegans-muscle-area-measurements-standardized-method-to-quantify

C. elegans Muscle Area Measurements: A Standardized Method to Quantify Striated Muscle Morphology

Source: Teoh, J. S. et al. Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in&#160;Caenorhabditis elegans.&#160;J. Vis. ...

epidermal laser wounding: inducing localized damage to examine repair processes in c. elegans

epidermal-laser-wounding-inducing-localized-damage-to-examine-repair

Epidermal Laser Wounding: Inducing Localized Damage to Examine Repair Processes in C. elegans

Source: Xu, S. and Chisholm, A. D. Methods for Skin Wounding and Assays for Wound Responses in&#160;C. elegans.&#160;J. Vis. Exp.&#160;(2014).
This video ...

fitc-dextran feeding: a method to quantify intestinal permeability in c. elegans

fitc-dextran-feeding-method-to-quantify-intestinal-permeability-c

FITC-Dextran Feeding: A Method to Quantify Intestinal Permeability in C. elegans

Source: Le, T. A. N., et al. Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of&#160;Caenorhabditis elegans.&#160;J. Vis. ...

nile red staining of c. elegans: a method to visualize lipid droplets in fixed animals

nile-red-staining-c-elegans-method-to-visualize-lipid-droplets-fixed

Nile Red Staining of C. elegans: A Method to Visualize Lipid Droplets in Fixed Animals

Source: Escorcia, W., et al. Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O ...

single cell dissociation of caenorhabditis elegans: a method to isolate live cells

single-cell-dissociation-caenorhabditis-elegans-method-to-isolate

Single Cell Dissociation of Caenorhabditis elegans: A Method to Isolate Live Cells

Source: Germany, E. M., et al. Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans. J. Vis. Exp. (2019).
This video describes a ...

ethanol fixation and dapi staining: a method to visualize dna in c. elegans

ethanol-fixation-dapi-staining-method-to-visualize-dna-c

Ethanol Fixation and DAPI Staining: A Method to Visualize DNA in C. elegans

Source: Clarke, E. K., et al. Manipulation of Ploidy in Caenorhabditis elegans. J. Vis. Exp. (2018).
Here, we describe a technique used to visualize ...

c. elegans blastomere dissection: a method to remove the eggshell and dissociate embryonic cells

c-elegans-blastomere-dissection-method-to-remove-eggshell-dissociate

C. elegans Blastomere Dissection: A Method to Remove the Eggshell and Dissociate Embryonic Cells

Source: Hsu, C. R., et al. In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated&#160;Caenorhabditis elegans&#160;Embryo Blastomeres and ...

enzymatic digestion and manual dissociation: a method to prepare c. elegans embryos for cell culture

enzymatic-digestion-manual-dissociation-method-to-prepare-c-elegans

Enzymatic Digestion and Manual Dissociation: A Method to Prepare C. elegans Embryos for Cell Culture

Source: Sangaletti, R. and Bianchi, L. A Method for Culturing Embryonic&#160;C. elegans&#160;Cells.&#160;J. Vis. Exp.&#160;(2013).
This video introduces a ...

rnai plating for c. elegans feeding: a technique to induce target dsrna expression in e. coli

rnai-plating-for-c-elegans-feeding-technique-to-induce-target-dsrna

RNAi Plating for C. elegans Feeding: A Technique to Induce Target dsRNA Expression in E. coli

Source: Kolundzic, E., et al. Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in&#160;C. ...

rnai feeding in liquid culture: a high-throughput method to knockdown gene expression in c. elegans

rnai-feeding-liquid-culture-high-throughput-method-to-knockdown-gene

RNAi Feeding in Liquid Culture: A High-Throughput Method to Knockdown Gene Expression in C. elegans

Source: Groh, N. et al. Methods to Study Changes in Inherent Protein Aggregation with Age in&#160;Caenorhabditis elegans.&#160;J. Vis. Exp.&#160;(2017).
...

enriching for males: maintaining a stock of male c. elegans

enriching-for-males-maintaining-a-stock-of-male-c-elegans

Enriching for Males: Maintaining a Stock of Male C. elegans

Source: Singaravelu, G., et al. Isolation and In vitro Activation of Caenorhabditis elegans Sperm. J. Vis. Exp. (2011).
Nematodes can be propagated easily ...

uv irradiation: a method to integrate extrachromosomal arrays

uv-irradiation-a-method-to-integrate-extrachromosomal-arrays

Encyclopedia Of Experiments - biology