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In this video, we demonstrate a surgical procedure to induce peripheral ischemia via femoral artery ligation in a rabbit. This method provides an effective experimental model for peripheral vascular disease research.
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Isolation of the femoral artery
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Figure 1: Induction of hind limb ischemia in rabbits through femoral artery ligation and excision. (A) Illustration of the vascular anatomy of the rabbit hind limb. Place ties at all the points marked to ligate the arteries. (B) Surgical field showing the cut down to the femoral artery prior to ligation. (C) Femoral arteries with ligations in place to induce hind limb ischemia.
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Name | Company | Catalog Number | Comments |
0.9% Sodium Chloride | Henry Schein Medical | 1537468 / 1531434 | 250 mL bag / 1000 mL irrigation btl |
10 mL Syringe | VWR | BD309695 | |
27G Needle | VWR | 89219-288 | |
Acepromazine | Patterson Veterinary | VEDC207 | |
Alfaxalone | Patterson Veterinary | 07-891-6051 | |
Buprenorphine SR | ZooPharm | ||
Hair Clippers | Amazon | B000CQZI3Q | Oster #40 blade |
Heating Pad | Cincinnati Subzero | 273 | |
Heating Pad Pump | Gaymar | Gaymar T/Pump | |
Hemostat | Fine Science Tools | 13009-12 | Curved Mosquito Hemostat |
Isoflurane | Henry Schein Medical | 1100734 | |
Ketamine | Patterson Veterinary | 856440301 | |
Lidocaine | McKesson Medical-Surgical | 239936 | |
Lidocaine/Prilocaine cream | McKesson Medical-Surgical | 761240 | |
Metzenbaum Scissors | Fine Science Tools | 14019-13 | |
Midazolam | Henry Schein Medical | 1215470 | |
Scalpel | Fine Science Tools | 10003-12 | |
Scalpel Blade | Fine Science Tools | 10015-00 | #15 blade |
Silk Sutures | Ethicon-Johnson & Johnson | A183H | 4-0 silk ties 18" |
Surgical Drapes | Gepco | 8204S | |
Weitlaner Retractor | Fine Science Tools | 17012-13 |
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This article has been published
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Source: Sligar, A. D., et al. Preclinical Model of Hind Limb Ischemia in Diabetic Rabbits. J. Vis. Exp. (2019).
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