Diagnostic Necropsy and Tissue Harvest
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN
Many animal experiments rely on final data collection time points that are gathered from the harvesting and testing of organs and tissues. The use of appropriate methods for the collection of organs and tissues can impact the quality of the samples and the analysis of the data that is gleaned for the testing of the tissues. The method of euthanasia of the animal can also impact the quality of the samples. This manuscript will outline proper necropsy techniques for rats.
1. External examination
A gross external examination of the body, which includes visual inspection of the body for lesions and masses, should be performed as the initial step in a necropsy. The hair coat should be examined for areas of hair loss. The teeth and nails are evaluated for excessive growth or wear. Any staining of the fur at the mouth, nares, eyes, anal, and genital openings should be noted. Tape tests, skin scraping, and pelt exams should be performed to detect external parasites (se
The final step in many research projects is the necropsy of the experimental animals. A detailed observation of external and internal structures followed by the collection of tissues for further analysis provides a great amount of research data. Proper techniques for tissue removal and preservation with the appropriate fixative solutions are essential for the correct interpretation of findings.
- Leary, S., Underwood, W., Anthony, R., Cartner, S., Corey, D., Grandin, T., et al. 2013. AVMA guidelines for the euthanasia of animals: 2013 edition.
- Parkinson, C.M., O'Brien, A., Albers, T.M., Simon, M.A., Clifford, C.B. and Pritchett-Corning, K.R. 2011. Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice. 54. e2966. 1-7.
- Fiette, L. and Slaoui, M. 2011. Necropsy and Sampling Procedures in Rodents. Drug Safety Evaluation: Methods and Protocols, 39-67.
- Youngson, R.M. 2005. Collins dictionary of Medicine fourth edition.
건너뛰기...
이 컬렉션의 비디오:
Now Playing
Diagnostic Necropsy and Tissue Harvest
Lab Animal Research
56.8K Views
Rodent Handling and Restraint Techniques
Lab Animal Research
167.5K Views
Basic Care Procedures
Lab Animal Research
26.6K Views
Fundamentals of Breeding and Weaning
Lab Animal Research
34.7K Views
Rodent Identification I
Lab Animal Research
53.1K Views
Rodent Identification II
Lab Animal Research
25.0K Views
Compound Administration I
Lab Animal Research
97.6K Views
Compound Administration II
Lab Animal Research
33.9K Views
Compound Administration III
Lab Animal Research
30.3K Views
Compound Administration IV
Lab Animal Research
49.9K Views
Blood Withdrawal I
Lab Animal Research
166.6K Views
Blood Withdrawal II
Lab Animal Research
70.7K Views
Anesthesia Induction and Maintenance
Lab Animal Research
49.1K Views
Considerations for Rodent Surgery
Lab Animal Research
21.8K Views
Sterile Tissue Harvest
Lab Animal Research
34.1K Views
JoVE 소개
Copyright © 2025 MyJoVE Corporation. 판권 소유