Name: individual lobe extraction from prostate: a technique to harvest individual prostate lobes from mouse prostate
Uploaded: 2023-04-30T13:28:50.000+00:00
Duration: 1 min 47 s
Description: Source: Nath, D. et al. Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models. J. ...

individual lobe extraction from prostate: a technique to harvest individual prostate lobes from mouse prostate

Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate

Place the tissue, dorsal side up, under a dissecting microscope so that the dorsal lobes, which resemble a butterfly&#39;s wings are visible. Holding each dorsal lobe with forceps, cut the tissue at its base with scissors to collect the dorsal lobes, and turn the tissue to the ventral side.
Collect the lateral lobes, which are small and usually wrap the urethra on the side and are wedged between the anterior, ventral, and dorsal lobes in the same manner. Collect the ventral lobes, which are larger than the lateral lobes and lie on the urethra ventrally. Then, cut and discard the urethra to harvest the anterior lobes.

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Research

JoVE Encyclopedia of Experiments

Cancer Research

Prostate Cancer

Animal model studies

EoE Sub Articles

eoe sub articles

All procedures involving animals have been reviewed by the local institutional animal care committee and the JoVE veterinary review board.
1. Gross Anatomy of the Prostate and Individual Lobe Microdissection
NOTE: This is depicted in Figures 1(i-n) and Figure 2
<ol>
	<li>Flip the tissue with a pair of blunt forceps so that the dorsal side faces up, showing the dorsal lobes, which resemble a butterfly&#8217;s wings.</li>
	<li>Collect the dorsal lobes by holding the lobe with forceps and snipping at the base with scissors (Figure 1j).</li>
	<li>Flip over the remaining tissue to the ventral side.</li>
	<li>Collect the lateral lobes, which are small and usually wrap the urethra on the side, which are wedged between the anterior, ventral, and dorsal lobes (Figure 1k).</li>
	<li>Next, collect the ventral lobes, which are larger than the lateral lobes and lie on the urethra ventrally (Figure 1l).</li>
	<li>Last, harvest the anterior lobes, the largest of the four, by cutting and discarding the urethra (Figure 1m).</li>
	<li>Process the tissue pieces according to experimental needs.</li>
</ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Mouse surgical instruments (Mouse Dissecting kit)</td>
			<td>World Precision Instruments</td>
			<td>MOUSEKIT</td>
			<td></td>
		</tr>
		<tr>
			<td>Dissection microscope</td>
			<td></td>
			<td></td>
			<td></td>
		</tr>
		<tr>
			<td>RPMI medium</td>
			<td>Thermofisher Scientific</td>
			<td>11875093</td>
			<td></td>
		</tr>
		<tr>
			<td>Dissection medium (DMEM + 10%FBS)</td>
			<td>Thermofisher Scientific</td>
			<td>11965-084</td>
			<td></td>
		</tr>
		<tr>
			<td>Fetal Bovine Serum</td>
			<td>Thermofisher Scientific</td>
			<td>10438018</td>
			<td></td>
		</tr>
		<tr>
			<td>PBS (Phosphate buffered saline)</td>
			<td>Thermofisher Scientific</td>
			<td>10010031</td>
			<td></td>
		</tr>
		<tr>
			<td>Collagenase</td>
			<td>Thermofisher Scientific</td>
			<td>17018029</td>
			<td>Make 10x stock (10mg/ml) in RPMI, filter sterilize, aliquot and store at -20 &#176;C</td>
		</tr>
		<tr>
			<td>Syringes and Needles</td>
			<td>Fisher Scientific</td>
			<td></td>
			<td></td>
		</tr>
	</tbody>
</table>

Source: Nath, D. et al. <a href="https://www.jove.com/video/58397" target="_blank">Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models.</a> J. Vis. Exp. (2018)
This video describes a technique to harvest individual lobes from the prostate gland of a mouse model. Individual lobes can then be used for histological analysis and cancer research.

<img alt="Figure 1" src="/files/ftp_upload/20394/20394_Figure1.jpg" /> 
Figure 1: Dissection of the mouse prostate. Step-by-step images for the dissection of the mouse prostate from the UGS: (a) removing fat, (b) removing the urinary bladder, (c) removing the vas deferens, (d) ventral view of the prostate with the urethra, (e) dorsal view of the prostate with the urethra, (f-...

Source: Nath, D. et al. Identification, Histological Characterization, and Dissection of Mouse Prostate Lobes for In Vitro 3D Spheroid Culture Models. J. ...

In rodents, the prostate gland is located around the base of the urinary bladder and surrounds the top portion of the urethra. The gland has four distinct pairs of lobes. Based on their anatomical orientation relative to the urinary bladder, they are called the ventral, lateral, anterior, and dorsal lobes.
The ventral lobes lie anterior to the urethra and inferior to the bladder. Two lateral prostate lobes, flanking the ventral lobes, reside on both sides of the urethra. The anterior lobes are translucent tubular structures attached to the seminal vesicles. The dorsal lobes, resembling butterfly wings, are positioned on the posterior side at the base of the seminal vesicles.
To harvest individual prostate lobes, begin by taking a mouse&#39;s urogenital system in a Petri dish. Use a dissecting microscope to distinguish the different lobes. Position the dorsal side to face upward. Locate the two dorsal lobes. Use scissors to excise them at their base. Now, invert the prostate tissue to face the ventral side. Locate the pair of lateral lobes. Use scissors to snip the lobes and separate them. Next, locate the ventral lobes and excise them. Lastly, separate the anterior lobes present at the top.

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Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate

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Individual Lobe Extraction from Prostate: A Technique to Harvest Individual Prostate Lobes from Mouse Prostate