JoVE Logo

로그인

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.

Establishing a Brain Tumor in an Organotypic Slice Co-culture Model

-- views • 1:01 min

내레이션 대본

Begin with an agarose-embedded mouse brain slice.

Place the brain slice on a membrane insert containing a culture medium.

Submerge the slice to ensure uniform exposure to nutrients.

Collect a small volume of the medium from the insert and transfer it to the bottom of the well.

Remove the excess medium from the insert.

Transfer the insert to a Petri dish containing the culture medium.

Carefully remove the agarose edges from the slice to make it accessible for subsequent interactions.

Place the insert in a new well containing the culture medium and incubate it to facilitate slice recovery.

Add human brain tumor cells to the slice and incubate.

Tumor cells interact with brain cells and the microenvironment within the slice, initiating multiplication and establishing a 3D co-culture model.

article

01:46

Establishing a Brain Tumor in an Organotypic Slice Co-culture Model

관련 동영상

65 Views

JoVE Logo

개인 정보 보호

이용 약관

정책

연구

교육

JoVE 소개

Copyright © 2025 MyJoVE Corporation. 판권 소유