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Navigational Behavior of Drosophila Larvae in Response to Optogenetic Stimulation of Olfactory Sensory Neurons

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Take control and transgenic larvae of the fruit fly Drosophila.

The transgenic larvae express a light-sensitive ion channel in the olfactory receptor neurons or ORNs. These neurons are part of the olfactory circuit, which detects odor stimuli and transmits signals to the brain, guiding larval movement in response to the stimuli.

Place the larvae on a dish with solidified agarose inside a behavior arena, and cover the dish.

Expose the larvae to a wavelength of light that stimulates the ion channels and record larval navigational behavior.

The light activates all-trans-retinal, a chromophore, which induces the opening of ion channels, known as optogenetic stimulation, and allows cation influx.

The influx generates action potentials that transmit signals along the olfactory circuit, impacting larval navigational behavior.

Transgenic larvae with light-sensitive ion channels exhibit a change in the distance traveled in a straight line before changing direction, known as run length, compared to the control larvae.

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Navigational Behavior of Drosophila Larvae in Response to Optogenetic Stimulation of Olfactory Sensory Neurons

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