electrophysiological recordings for assessing neuronal regenerations in co-cultured spinal cord slices

Electrophysiological Recordings For Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices

Put a Petri dish with a coated microelectrode array inside under a stereomicroscope. Bring the array into focus, and center a six-microliter droplet of chicken plasma on the electrode array. Using a small spatula, carefully slide two spinal cord sections with their ventral sides facing each other into the plasma droplet.
Next, add eight microliters of thrombin around the chicken plasma droplet. Then, use the pipette tip to carefully mix and spread the chicken plasma and thrombin mixture. Just before the chicken plasma and thrombin mixture becomes too stringy and starts to coagulate, aspirate the excess liquid. Then, cap the Petri dish and place it in a humidified chamber.
Place the chamber inside an incubator at 37 degrees Celsius for about an hour. After incubation, carefully add 10 microliters of nutrient medium to the sample. Cap the Petri dish and place it back into the incubator for an additional 45 minutes. Next, place each of the multi-electrode array culture assemblies into a roller tube and add three milliliters of nutrient medium. Close the lid tightly and place the roller tube into the roller drum. Rotate the drum at 1 to 2 RPM in the incubator at 37 degrees Celsius.
Using sterile rubber-tipped forceps, remove the multi-electrode array culture assemblies from the roller tube and place them into a Petri dish under a stereomicroscope. Bring the tissue into focus and verify that the two slices are fused. Next, hold the assembly steady and place a scalpel blade in the groove of the multielectrode array close to the tissue slices. Hold the scalpel rather horizontally and then lift the scalpel handle up, but let the scalpel blade stay in the groove of the array in such a way that the blade rolls from base to tip, cutting through the tissue, covering the groove.
Sever any residual tissue connections with a 25-gauge needle tip if necessary. Work only in the area within the groove, and do not touch the tender edges. Put the multi-electrode array culture assemblies back into the roller tube and add three milliliters of fresh nutrient medium to the cultures. Then, place the roller tube back onto the roller drum in the incubator at 37 degrees Celsius.
Mount a multi-electrode array culture assembly into a recording chamber and apply about 500 microliters of extracellular solution to the array. Then, mount the assembly on the microscope. Wait 10 minutes for the system to stabilize, and then, record basic spontaneous activity from each of the activity-detecting electrodes for 10 minutes. Repeat the recordings a total of two times.
To ensure stable extracellular conditions, exchange the extracellular solution after every recording session. If desired, disinhibit the network by applying extracellular solution containing one micromolar of strychnine and 10 micromolar of gabazine and wait for at least two minutes before recording the electrical activity.

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All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Mounting Spinal Cord Tissue Slices on MEAs
<ol>
	<li>Warm up sterile nutrient medium in the incubator (37 &#176;C, lightly unscrewed lid for oxygenation).</li>
	<li>Position the multi-electrode array or MEA with sterile tweezers with rubber-covered tips at room temperature (RT) in the petri dish under a stereo microscope with the electrode array in focus. Center a 6 &#181;l droplet of chicken plasma on the clean, dust-free, and sterile electrode array. Using a small spatula, carefully slide two spinal cord sections with ventral sides facing each other into the plasma droplet. Do not touch the electrodes with the spatula.</li>
	<li>Add 8 &#181;l of thrombin around the chicken plasma droplet. Using the thrombin pipette tip, carefully mix and spread the chicken plasma/thrombin mixture around the two slices. Again, do not touch the brittle electrode array directly. Just before coagulation, aspirate excess chicken plasma/thrombin.</li>
	<li>Cap the petri dish to retain high humidity while the MEA/culture assembly sits for about 1 hr in a humidified chamber inside the incubator at 37 &#176;C.</li>
	<li>Carefully add 10 &#181;l of nutrient medium to the culture chamber, cap the petri dish, and put back into the incubator for about 30 min.</li>
	<li>Place each MEA/culture assembly with sterile, rubber-covered tips into a roller tube, add 3 ml of nutrient medium, and tightly close the lid. Place the roller tube in the roller drum, rotating at 1 - 2 rpm in the incubator in a 5% CO2-containing atmosphere at 37 &#176;C.</li>
	<li>Change half of the nutrient medium after 7 days in vitro (DIV) and afterward 1 - 2 times per week.</li>
</ol>
2. Mechanical Lesions
<ol>
	<li>Take the MEA/culture assembly with sterile, rubber-covered tips out of the roller tube and place in a petri dish without nutrient medium under a stereo microscope with the tissue in focus. Visually verify that the two slices are fused.</li>
	<li>Hold the MEA/culture assembly steady by placing tweezers with rubber-covered tips on the MEA.</li>
	<li>Place a scalpel blade in the groove of the MEA close to the tissue slices. Hold the scalpel rather horizontally.</li>
	<li>Lift the scalpel handle up but let the scalpel blade stay in the groove of the MEA in such a way that the blade &#34;rolls&#34; from base to tip and thereby cuts through the tissue covering the groove.</li>
	<li>Severe any residual tissue connections with a 25 G needle tip if necessary. Work only in the area within the groove and do not touch the tender edges.</li>
	<li>Put the MEA/culture assembly back into the roller tube. Provide 3 ml of fresh nutrient medium to the cultures and place them back into the roller drum in the incubator.</li>
</ol>
3. Electrophysiological Recordings of Spontaneous Activity
<ol>
	<li>To investigate functional regeneration among the two spinal cord slices after the chosen number of DIV, mount the MEA/culture assembly in a recording chamber on a microscope and apply about 500 &#181;l extracellular solution (see materials list).</li>
	<li>Wait 10 min before the first recording to allow the system to stabilize.</li>
	<li>Record basic spontaneous activity 2x for about 10 min from each activity-detecting electrode of the MEA at RT.</li>
	<li>To ensure stable, extracellular conditions, exchange extracellular solution after every recording session.</li>
	<li>To disinhibit the network, apply an extracellular solution containing strychnine (1 &#956;M) and gabazine (10 &#956;M).&#160; Wait for at least 2 min before starting the recording.</li>
</ol>
4. Data Analysis
NOTE: For the detection of the extracellularly recorded action potentials, use a detector based on standard deviations and a subsequent discriminator for each electrode.
<ol>
	<li>Display the detected neuronal activity of each electrode in a raster plot according to standard procedures (Figure 1F).</li>
	<li>Determine and display the total network activity for each slice by summarizing the number of events in the appropriate channels within a sliding window of 10 msec, shifted by 1 msec steps (Figure 1G).</li>
	<li>Detect in each slice individual bursts (clusters of activity that appear on several electrodes). Therefore, set a minimal peak activity threshold in the corresponding total network activity and define each burst start and burst end. For example, an appropriate threshold is 25% of the average burst amplitude, and a burst start can be defined as being the first event in a time window of at least 5 msec, and a burst end by being the last event in a time window of at least 25 msec.</li>
	<li>To quantify functional regeneration, calculate the percentage of synchronized bursts between the two slices. To do this, calculate the latency between a burst in one slice and the following burst in the other slice. 
	NOTE: A burst pair is termed &#34;synchronized&#34; when the latency is smaller than the average burst length. Especially in co-cultures with a lot of activity, the possibility exists that both sides coincidentally initiate a burst in the chosen latency window. This fact has to be taken into account in the quantification of the percentage of synchronized bursts.</li>
</ol>

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>Planar multielectrode array</td>
			<td>Qwane Biosciences</td>
			<td></td>
			<td>custom-made according to the design of our lab</td>
		</tr>
		<tr>
			<td>Nutrient medium</td>
			<td></td>
			<td></td>
			<td>For 100 ml</td>
		</tr>
		<tr>
			<td>Dulbeccos modified Eagle&#39;s medium</td>
			<td>Gibco</td>
			<td>31966-021</td>
			<td>80 ml</td>
		</tr>
		<tr>
			<td>Horse serum</td>
			<td>Gibco</td>
			<td>26050-070</td>
			<td>10 ml</td>
		</tr>
		<tr>
			<td>distilled, sterile water</td>
			<td></td>
			<td></td>
			<td>10 ml</td>
		</tr>
		<tr>
			<td>Nerve growth factor-7S [5ng/mL]</td>
			<td>Sigma-Aldrich</td>
			<td>N0513</td>
			<td>200 &#181;l</td>
		</tr>
		<tr>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>&#160; &#160;</td>
			<td>reconstituted in wash solution with 1% BSA</td>
		</tr>
		<tr>
			<td>Extracellular matrix gel</td>
			<td>BD Biosciences</td>
			<td>356230</td>
			<td>Must stay cold at all times; dilute 1:50 with medium optimized for prenatal and embryonic neurons</td>
		</tr>
		<tr>
			<td>Extracellular solution (pH 7.4)</td>
			<td></td>
			<td></td>
			<td>[mM]</td>
		</tr>
		<tr>
			<td>NaCl</td>
			<td></td>
			<td></td>
			<td>145</td>
		</tr>
		<tr>
			<td>KCl</td>
			<td></td>
			<td></td>
			<td>4</td>
		</tr>
		<tr>
			<td>MgCl2</td>
			<td></td>
			<td></td>
			<td>1</td>
		</tr>
		<tr>
			<td>CaCl2</td>
			<td></td>
			<td></td>
			<td>2</td>
		</tr>
		<tr>
			<td>HEPES</td>
			<td></td>
			<td></td>
			<td>5</td>
		</tr>
		<tr>
			<td>Na-pyruvate</td>
			<td></td>
			<td></td>
			<td>2</td>
		</tr>
		<tr>
			<td>Glucose</td>
			<td></td>
			<td></td>
			<td>5</td>
		</tr>
		<tr>
			<td>Gabazine</td>
			<td>Sigma-Aldrich</td>
			<td>SR-95531</td>
			<td></td>
		</tr>
		<tr>
			<td>Micropipette</td>
			<td>World Precision Instruments, Inc.</td>
			<td>MF28G-5</td>
			<td></td>
		</tr>
		<tr>
			<td>Strychnine</td>
			<td>Sigma-Aldrich</td>
			<td>S0532</td>
			<td>toxic</td>
		</tr>
		<tr>
			<td>Tissue culture flat tube 10 (= roller tube)</td>
			<td>Techno Plastic Products AG</td>
			<td>91243</td>
			<td></td>
		</tr>
	</tbody>
</table>

Source: Heidemann, M., et al. <a href="https://app.jove.com/v/53121">Investigating Functional Regeneration in Organotypic Spinal Cord Co-cultures Grown on Multi-electrode Arrays.</a> J. Vis. Exp. (2015).
This video presents electrophysiological recordings from organotypic co-cultures of spinal cord slices, captured using a multi-electrode array or MEA device. These recordings assess neuronal regeneration between the slices. The synchronized electrical activity observed confirms successful neural regeneration.

<img alt="Figure 1" src="/files/ftp_upload/22714/22714_Figure1.jpg" /> 
Figure 1. Display and Analysis of Spontaneous Activity. (A) Diagram of a MEA. The platinum-covered electrodes are depicted in black, the transparent wires in red, and the groove in the middle of the MEA in yellow. (B) Close-up of the electrode array located in the center of the MEA. (C) Bright-field image of an 8 DIV old culture. The slices have grown and fused along the sides facing each other. ...

All procedures involving animal samples have been reviewed and approved by the appropriate animal ethical review committee.
1. Mounting Spinal Cord Tissue ...

Take a polymer-coated multi-electrode array or MEA, with co-cultured embryonic spinal cord slices positioned in two separate zones, allowing for simultaneous recording from both slices.
The slice shows regenerated neural connections in the previously lesioned area.
Place the MEA in a recording chamber containing an extracellular solution rich in ions and nutrients to maintain neuronal survival.
Allow the system to stabilize, facilitating neuron adaptation and communication.
Neurons communicate via electrical signals called action potentials, which are recorded by the nearby electrodes in the MEA.&#160;
Individual signals from each electrode are combined, producing an electrical signal from each slice.
Regularly replace the extracellular solution to maintain neuron stability.
Add an extracellular solution with inhibitors that block inhibitory neurotransmitter receptors on neuronal membranes, keeping neurons active for a longer duration.
Compare the electrical activity from both slices. A synchronized electrical signal between the two slices confirms the synaptic connections and successful neural regeneration.

16 조회수. 전기생리학적 기록(Electrophysiological Recordings for Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices)

비디오: 전기생리학적 기록(Electrophysiological Recordings for Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices) - 실험

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes (hPSC-CMs) Using Multi-electrode Arrays (MEAs)

Cardiomyocytes can now be derived with high efficiency from both human embryonic and human induced-Pluripotent Stem Cells (hPSC). hPSC-derived cardiomyocytes (hPSC-CMs) are increasingly recognized as having great value for modeling cardiovascular diseases in humans, especially arrhythmia syndromes. They have also demonstrated relevance as in vitro systems for predicting drug responses, which makes them potentially useful for drug-screening and discovery, safety pharmacology and perhaps eventually for personalized medicine. This would be facilitated by deriving hPSC-CMs from patients or susceptible individuals as hiPSCs. For all applications, however, precise measurement and analysis of hPSC-CM electrical properties are essential for identifying changes due to cardiac ion channel mutations and/or drugs that target ion channels and can cause sudden cardiac death. Compared with manual patch-clamp, multi-electrode array (MEA) devices offer the advantage of allowing medium- to high-throughput recordings. This protocol describes how to dissociate 2D cell cultures of hPSC-CMs to small aggregates and single cells and plate them on MEAs to record their spontaneous electrical activity as field potential. Methods for analyzing the recorded data to extract specific parameters, such as the QT and the RR intervals, are also described here. Changes in these parameters would be expected in hPSC-CMs carrying mutations responsible for cardiac arrhythmias and following addition of specific drugs, allowing detection of those that carry a cardiotoxic risk.

Electrophysiological Analysis of human Pluripotent Stem Cell-derived Cardiomyocytes hPSC-CMs Using Multi-electrode Arrays MEAs

Electrophysiological characterization of cardiomyocytes derived from human Pluripotent Stem Cells (hPSC-CMs) is crucial for cardiac disease modeling and for determining drug responses. This protocol provides the necessary information to dissociate and plate hPSC-CMs on multi-electrode arrays, measure their field potential, and a method for analyzing QT and RR intervals.

다중 전극 배열 (MEAs)을 이용한 인간 다 능성 줄기 세포 유래 심근 세포 (hPSC-CMs)의 전기 생리 학적 분석

Cardiomyocytes can now be derived with high efficiency from both human embryonic and human induced-Pluripotent Stem Cells (hPSC). hPSC-derived ...

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JoVE Journal

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Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Bis-phenols, such as bis-phenol A (BPA) and bis-phenol-S (BPS), are polymerizing agents widely used in the production of plastics and numerous everyday products. They are classified as endocrine disrupting compounds (EDC) with estradiol-like properties. Long-term exposure to EDCs, even at low doses, has been linked with various health defects including cancer, behavioral disorders, and infertility, with greater vulnerability during early developmental periods. To study the effects of BPA on the development of neuronal function, we used an in vitro neuronal network derived from the early chick embryonic brain as a model. We found that exposure to BPA affected the development of network activity, specifically spiking activity and synchronization. A change in network activity is the crucial link between the molecular target of a drug or compound and its effect on behavioral outcome. Multi-electrode arrays are increasingly becoming useful tools to study the effects of drugs on network activity in vitro. There are several systems available in the market and, although there are variations in the number of electrodes, the type and quality of the electrode array and the analysis software, the basic underlying principles, and the data obtained is the same across the different systems. Although currently limited to analysis of two-dimensional in vitro cultures, these MEA systems are being improved to enable in vivo network activity in brain slices. Here, we provide a detailed protocol for embryonic exposure and recording neuronal network activity and synchrony, along with representative results.

Exposure to environmental toxins can acutely impact developing embryos. Endocrine disrupting chemicals such as bisphenols are known to adversely affect the nervous system. Here we describe a protocol using an in vitro vertebrate (chick embryo) neuronal network model to study the functional impact of toxin exposure on early embryos.

내 분 비 방해 다중 전극 어레이 사용 하 여 척추 신경 네트워크 기능 개발에 화합물의 효과의 평가

Bis-phenols, such as bis-phenol A (BPA) and bis-phenol-S (BPS), are polymerizing agents widely used in the production of plastics and numerous everyday ...

Multi-electrode Array Recordings of Human Epileptic Postoperative Cortical Tissue

Epilepsy, affecting about 1% of the population, comprises a group of neurological disorders characterized by the periodic occurrence of seizures, which disrupt normal brain function. Despite treatment with currently available antiepileptic drugs targeting neuronal functions, one third of patients with epilepsy are pharmacoresistant. In this condition, surgical resection of the brain area generating seizures remains the only alternative treatment. Studying human epileptic tissues has contributed to understand new epileptogenic mechanisms during the last 10 years. Indeed, these tissues generate spontaneous interictal epileptic discharges as well as pharmacologically-induced ictal events which can be recorded with classical electrophysiology techniques. Remarkably, multi-electrode arrays (MEAs), which are microfabricated devices embedding an array of spatially arranged microelectrodes, provide the unique opportunity to simultaneously stimulate and record field potentials, as well as action potentials of multiple neurons from different areas of the tissue. Thus MEAs recordings offer an excellent approach to study the spatio-temporal patterns of spontaneous interictal and evoked seizure-like events and the mechanisms underlying seizure onset and propagation. Here we describe how to prepare human cortical slices from surgically resected tissue and to record with MEAs interictal and ictal-like events ex vivo.

We here describe how to perform multi-electrode array recordings of human epileptic cortical tissue. Epileptic tissue resection, slice preparation and multi-electrode array recordings of interictal and ictal events are demonstrated in detail.

인간의 간질 수술 후 두피 조직의 다중 전극 어레이 레코딩

Epilepsy, affecting about 1% of the population, comprises a group of neurological disorders characterized by the periodic occurrence of seizures, which ...

Electrophysiological Recordings For Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices

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Electrophysiological Recordings For Assessing Neuronal Regenerations in Co-cultured Spinal Cord Slices