February 10th, 2016
•Filamentous actin (F-actin) plays an important role in spinogenesis, synaptic plasticity, and synaptic stability. Quantification of F-actin puncta is therefore a useful tool to study the integrity of synaptic structures. This protocol describes the procedures of quantifying F-actin puncta labeled with Phalloidin in low-density primary cortical neuronal cultures.
Tags
Related Videos
Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons
Labeling F-actin Barbed Ends with Rhodamine-actin in Permeabilized Neuronal Growth Cones
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons
Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia
In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons
Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines
Copyright © 2024 MyJoVE Corporation. 판권 소유