Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

DOI :

10.3791/57664-v

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June 13th, 2018

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8,704 Views

Ruoji Zhou^1,2, An Xu¹, Jian Tu^1,3, Mo Liu¹, Julian A Gingold⁴, Ruiying Zhao¹, Dung-Fang Lee^1,2,5,6

¹Department of Integrative Biology and Pharmacology, McGovern Medical School, The University of Texas Health Science Center at Houston, ²Graduate School of Biomedical Sciences, The University of Texas MD Anderson Cancer Center UTHealth, ³Department of Musculoskeletal Oncology, The First Affiliated Hospital of Sun Yat-sen University, ⁴Women's Health Institute, Cleveland Clinic Foundation, ⁵Center for Stem Cell and Regenerative Medicine, The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases, The University of Texas Health Science Center at Houston, ⁶Center for Precision Health, School of Biomedical Informatics and School of Public Health, The University of Texas Health Science Center at Houston

Chapters

Here, we present a protocol for the generation of induced pluripotent Stem Cells (iPSCs) from Li-Fraumeni Syndrome (LFS) patient derived fibroblasts, differentiation of iPSCs via mesenchymal stem cells (MSCs) to osteoblasts, and modeling in vivo tumorigenesis using LFS patient-derived osteoblasts.

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