March 14th, 2019
•Cell-free expression systems are powerful and cost-efficient tools for the high-throughput synthesis and screening of important proteins. Here, we describe the preparation of cell-free protein expression system using Vibrio natriegens for the rapid protein production using plasmid DNA, linear DNA, and mRNA template.
Related Videos
High-throughput Protein Expression Generator Using a Microfluidic Platform
Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study
Sealable Femtoliter Chamber Arrays for Cell-free Biology
Studying Protein Function and the Role of Altered Protein Expression by Antibody Interference and Three-dimensional Reconstructions
Synthesis of Infectious Bacteriophages in an E. coli-based Cell-free Expression System
A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression
High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer
OnePot PURE Cell-Free System
Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
Copyright © 2024 MyJoVE Corporation. 판권 소유