August 4th, 2021
•As currently implemented, optogenetics in non-human primates requires injection of viral vectors into the brain. An optimal injection method should be reliable and, for many applications, capable of targeting individual sites of arbitrary depth that are readily and unambiguously identified in postmortem histology. An injection method with these properties is presented.
Related Videos
DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices
Large-scale Recording of Neurons by Movable Silicon Probes in Behaving Rodents
A Procedure for Implanting Organized Arrays of Microwires for Single-unit Recordings in Awake, Behaving Animals
Simultaneous Electrophysiological Recording and Micro-injections of Inhibitory Agents in the Rodent Brain
Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy
Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
Optogenetics Identification of a Neuronal Type with a Glass Optrode in Awake Mice
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
In Vivo Intracerebral Stereotaxic Injections for Optogenetic Stimulation of Long-Range Inputs in Mouse Brain Slices
In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal
Copyright © 2024 MyJoVE Corporation. 판권 소유