JoVE Table of Contents: Issue 192, February 2023

A Simple Technique to Assay Locomotor Activity in <em>Drosophila</em>

A Simple Technique to Assay Locomotor Activity in Drosophila

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Journal (Behavior)

A Smartphone-Based Imaging Method for <em>C. elegans</em> Lawn Avoidance Assay

A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay

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Journal (Behavior)

|

Methods Collections

Rapid Development of Cell State Identification Circuits with Poly-Transfection

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Journal (Bioengineering)

|

Methods Collections

Real-Time Void Spot Assay

|

Journal (Behavior)

|

Methods Collections

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

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Journal (Biochemistry)

|

Methods Collections

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

|

Journal (Biochemistry)

|

Methods Collections

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

|

Journal (Bioengineering)

|

Methods Collections

Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

|

Journal (Bioengineering)

|

Methods Collections

Simple Continuous Glucose Monitoring in Freely Moving Mice

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Journal (Biology)

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

|

Journal (Biology)

|

Methods Collections

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

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Journal (Biology)

|

Methods Collections

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of <em>Mycoplasma pneumoniae</em>

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

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Journal (Biology)

|

Methods Collections

Live Imaging of <em>Arabidopsis</em> Pollen Tube Reception and Double Fertilization Using the Semi-<em>In Vitro Cum Septum</em> Method

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

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Journal (Biology)

|

Methods Collections

Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance

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Journal (Biology)

|

Methods Collections

Autofluorescence Imaging to Evaluate Red Algae Physiology

|

Journal (Biology)

|

Methods Collections

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

|

Journal (Biology)

|

Methods Collections

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

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Journal (Cancer Research)

Glioblastoma Relapse Post-Resection Model for Therapeutic Hydrogel Investigations

|

Journal (Cancer Research)

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

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Journal (Cancer Research)

|

Methods Collections

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids <em>via</em> Immunofluorescence Assays

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

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Journal (Cancer Research)

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Methods Collections

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

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Journal (Cancer Research)

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors <em>Arid1a</em> and <em>Pten</em> in Mice

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

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Journal (Cancer Research)

Translational Orthotopic Models of Glioblastoma Multiforme

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Journal (Cancer Research)

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Methods Collections

Establishment and Culture of Patient-Derived Breast Organoids

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Journal (Cancer Research)

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Methods Collections

Gene Expression Analyses in Human Follicles

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Journal (Developmental Biology)

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Methods Collections

Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos

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Journal (Developmental Biology)

Measuring Embryonic Viability and Brood Size in <em>Caenorhabditis elegans</em>

Measuring Embryonic Viability and Brood Size in Caenorhabditis elegans

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Journal (Genetics)

The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using <em>Drosophila melanogaster</em>

The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using Drosophila melanogaster

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Journal (Genetics)

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Methods Collections

Simplified Whole Body Plethysmography to Characterize Lung Function During Respiratory Melioidosis

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Journal (Immunology and Infection)

Experimental Model to Evaluate Resolution of Pneumonia

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Journal (Immunology and Infection)

|

Methods Collections

Induction of Periodontitis <em>via</em> a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

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Journal (Immunology and Infection)

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Methods Collections

Assessment of Intestinal Transcytosis of Neonatal <em>Escherichia coli</em> Bacteremia Isolates

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates

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Journal (Immunology and Infection)

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Methods Collections

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

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Journal (Immunology and Infection)

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Methods Collections

Full-Endoscopic Interlaminar Approach for Decompression of Lateral Recess Stenosis

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Journal (Medicine)

|

Methods Collections

Behavioral and Network Pharmacology-Based Analyses for the Traditional Mongolian Medicine Zadi-5 in a Rat Model of Depression

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Journal (Medicine)

|

Methods Collections

A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts

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Journal (Medicine)

Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

|

Journal (Medicine)

|

Methods Collections

A Personalized 3D-Printed Model for Preoperative Evaluation in Thyroid Surgery

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Journal (Medicine)

|

Methods Collections

Mechanical Control of Relaxation Using Intact Cardiac Trabeculae

|

Journal (Medicine)

|

Methods Collections

Three-Dimensional Preoperative Virtual Planning in Derotational Proximal Femoral Osteotomy

|

Journal (Medicine)

|

Methods Collections

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

|

Journal (Medicine)

|

Methods Collections

Meta-Analysis of the Effectiveness and Safety of Shugan Jieyu Capsules for the Treatment of Insomnia

|

Journal (Medicine)

|

Methods Collections

Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning

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Journal (Neuroscience)

Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons

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Journal (Neuroscience)

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Methods Collections

Whole Central and Peripheral Nervous System Mice Dissection

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Journal (Neuroscience)

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

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Journal (Medicine)

|

Methods Collections

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

|

Journal (Biochemistry)

|

Methods Collections

Sonodynamic Therapy for the Treatment of Glioblastoma Multiforme in a Mouse Model Using a Portable Benchtop Focused Ultrasound System

|

Journal (Bioengineering)

|

Methods Collections

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

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Journal (Biology)

<em>Ex Vivo</em> Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures

Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures

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Journal (Biology)

|

Methods Collections

Characterizing Epithelial Wound Healing <em>In Vivo</em> Using the Cnidarian Model Organism <em>Clytia hemisphaerica</em>

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

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Journal (Biology)

|

Methods Collections

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

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Journal (Biology)

|

Methods Collections

A Simple Protocol for Mapping the Plant Root System Architecture Traits

|

Journal (Biology)

|

Methods Collections

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

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Journal (Biology)

|

Methods Collections

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

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Journal (Bioengineering)

|

Methods Collections

Setup of Consumer Wearable Devices for Exposure and Health Monitoring in Population Studies

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Journal (Bioengineering)

|

Methods Collections

High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle

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Journal (Biology)

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Methods Collections

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

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Journal (Biology)

|

Methods Collections

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

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Journal (Biology)

|

Methods Collections

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

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Journal (Biology)

|

Methods Collections

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

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Journal (Biology)

|

Methods Collections

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

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Journal (Biology)

|

Methods Collections

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

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Journal (Cancer Research)

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Methods Collections

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

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Journal (Cancer Research)

<em>In Vivo</em> Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

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Journal (Cancer Research)

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Methods Collections

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

|

Journal (Cancer Research)

|

Methods Collections

Development of a <sup>68</sup>Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Development of a ⁶⁸Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

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Journal (Cancer Research)

|

Methods Collections

Spontaneous Murine Model of Anaplastic Thyroid Cancer

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Journal (Cancer Research)

|

Methods Collections

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

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Journal (Cancer Research)

|

Methods Collections

Synthesis of Single-Crystalline Core-Shell Metal-Organic Frameworks

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Journal (Chemistry)

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Methods Collections

A Scalable Balz-Schiemann Reaction Protocol in a Continuous Flow Reactor

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Journal (Chemistry)

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Methods Collections

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

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Journal (Chemistry)

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Methods Collections

3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss <em>Physcomitrium patens</em>

3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens

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Journal (Developmental Biology)

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Methods Collections

<em>In Vitro</em> Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

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Journal (Developmental Biology)

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Methods Collections

Fabrication of a Low-Cost, Fiber-Coupled, and Air-Spaced Fabry-Pérot Etalon

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Journal (Engineering)

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

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Journal (Environment)

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Methods Collections

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

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Journal (Genetics)

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Methods Collections

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

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Journal (Genetics)

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

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Journal (Immunology and Infection)

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Methods Collections

Gene Editing of Primary Rhesus Macaque B Cells

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Journal (Immunology and Infection)

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Methods Collections

A Mouse Model of Chronic Liver Fibrosis for the Study of Biliary Atresia

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Journal (Immunology and Infection)

Single-Anastomosis Duodeno-Ileal Bypass with Sleeve Gastrectomy Model in Mice

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Journal (Medicine)

Spinal Shortening Surgery for Lumbosacral Nerve Bowstring Disease: A Surgical Technique

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Journal (Medicine)

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Methods Collections

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

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Journal (Medicine)

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Methods Collections

Laparoscopic Common Bile Duct Exploration in Patients with a Previous History of Biliary Tract Surgery

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Journal (Medicine)

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Methods Collections

Image-Guided Optical Coherence Tomography to Assess Structural Changes in Rodent Retinas

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Journal (Medicine)

A Modified Technique for Arteriovenous Fistula Construction in Rabbits

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Journal (Medicine)

Real-Time Monitoring and Modulation of Blood Pressure in a Rabbit Model of Ischemic Stroke

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Journal (Medicine)

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Methods Collections

Point-Of-Care Ultrasound Screening for Proximal Lower Extremity Deep Venous Thrombosis

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Journal (Medicine)

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Methods Collections

Isolation and Culture of Human Adipose-Derived Stem Cells With an Innovative Xenogeneic-Free Method for Human Therapy

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Journal (Medicine)

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Methods Collections

Formation of Dispersible Taohong Siwu Tablets

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Journal (Medicine)

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Methods Collections

Intubation, Central Venous Catheter, and Arterial Line Placement in Swine for Translational Research in Abdominal Transplantation Surgery

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Journal (Medicine)

Micro-Ultrasound Guided Transperineal Prostate Biopsy: A Clinic-Based Procedure

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

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Journal (Medicine)

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Methods Collections

<em>In Vivo</em> Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

In Vivo Calcium Imaging of Neuronal Ensembles in Networks of Primary Sensory Neurons in Intact Dorsal Root Ganglia

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Journal (Neuroscience)

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Methods Collections

In Vitro Myelination of Peripheral Axons in a Coculture of Rat Dorsal Root Ganglion Explants and Schwann Cells

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Journal (Neuroscience)

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Methods Collections

Modified Experimental Conditions for Noise-Induced Hearing Loss in Mice and Assessment of Hearing Function and Outer Hair Cell Damage

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Journal (Neuroscience)

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Methods Collections

Inhoudsopgave

Archiveren: Issue 192, February 2023

GA NAAR

A Simple Technique to Assay Locomotor Activity in Drosophila

A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay

Rapid Development of Cell State Identification Circuits with Poly-Transfection

Real-Time Void Spot Assay

An Optimized Quantitative Pull-Down Analysis of RNA-Binding Proteins Using Short Biotinylated RNA

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence (TIRF) Microscopy

A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes

Reconstituting Cytoarchitecture and Function of Human Epithelial Tissues on an Open-Top Organ-Chip

Simple Continuous Glucose Monitoring in Freely Moving Mice

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

Cell-Specific Paired Interrogation of the Mouse Ovarian Epigenome and Transcriptome

Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae

Live Imaging of Arabidopsis Pollen Tube Reception and Double Fertilization Using the Semi-In Vitro Cum Septum Method

Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance

Autofluorescence Imaging to Evaluate Red Algae Physiology

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Intracranial Cannula Implantation for Serial Locoregional Chimeric Antigen Receptor (CAR) T Cell Infusions in Mice

Glioblastoma Relapse Post-Resection Model for Therapeutic Hydrogel Investigations

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

Generation of 3D Tumor Spheroids for Drug Evaluation Studies

Non-Invasive Ultrasound Assessment of Endometrial Cancer Progression in Pax8-Directed Deletion of the Tumor Suppressors Arid1a and Pten in Mice

Translational Orthotopic Models of Glioblastoma Multiforme

Establishment and Culture of Patient-Derived Breast Organoids

Gene Expression Analyses in Human Follicles

Efficient Vascularization of Kidney Organoids through Intracelomic Transplantation in Chicken Embryos

Measuring Embryonic Viability and Brood Size in Caenorhabditis elegans

The Serial Anesthesia Array for the High-Throughput Investigation of Volatile Agents Using Drosophila melanogaster

Simplified Whole Body Plethysmography to Characterize Lung Function During Respiratory Melioidosis

Experimental Model to Evaluate Resolution of Pneumonia

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates

Isolation of Low Endotoxin Content Extracellular Vesicles Derived from Cancer Cell Lines

Full-Endoscopic Interlaminar Approach for Decompression of Lateral Recess Stenosis

Behavioral and Network Pharmacology-Based Analyses for the Traditional Mongolian Medicine Zadi-5 in a Rat Model of Depression

A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts

Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

A Personalized 3D-Printed Model for Preoperative Evaluation in Thyroid Surgery

Mechanical Control of Relaxation Using Intact Cardiac Trabeculae

Three-Dimensional Preoperative Virtual Planning in Derotational Proximal Femoral Osteotomy

A Protocol for Constructing a Rat Wound Model of Type 1 Diabetes

Meta-Analysis of the Effectiveness and Safety of Shugan Jieyu Capsules for the Treatment of Insomnia

Enrichment of Adult Mouse Dorsal Root Ganglia Neuron Cultures by Immunopanning

Measuring Axonal Cargo Transport in Mouse Primary Cortical Cultured Neurons

Whole Central and Peripheral Nervous System Mice Dissection

Clinical Application of 24 G Cannula Needle and 3-0 Polypropylene Suture in Vas Deferens Exploration

Purification of Active Photosystem I-Light Harvesting Complex I from Plant Tissues

Sonodynamic Therapy for the Treatment of Glioblastoma Multiforme in a Mouse Model Using a Portable Benchtop Focused Ultrasound System

Single-Cell Suspension Preparation from Nile Tilapia Intestine for Single-Cell Sequencing

Ex Vivo Expansion and Genetic Manipulation of Mouse Hematopoietic Stem Cells in Polyvinyl Alcohol-Based Cultures

Characterizing Epithelial Wound Healing In Vivo Using the Cnidarian Model Organism Clytia hemisphaerica

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

A Simple Protocol for Mapping the Plant Root System Architecture Traits

Culture of Piglet Intestinal 3D Organoids from Cryopreserved Epithelial Crypts and Establishment of Cell Monolayers

Three-Dimensional Culture of Vascularized Thermogenic Adipose Tissue from Microvascular Fragments

Setup of Consumer Wearable Devices for Exposure and Health Monitoring in Population Studies

High-Resolution Fluoro-Respirometry of Equine Skeletal Muscle

Establishment, Maintenance, Differentiation, Genetic Manipulation, and Transplantation of Mouse and Human Lacrimal Gland Organoids

Microvascular and Macrovascular Endothelial Cell Isolation and Purification from Lung-Derived Samples

Following the Dynamics of Structural Variants in Experimentally Evolved Populations

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Real-Time Analysis of Bioenergetics in Primary Human Retinal Pigment Epithelial Cells Using High-Resolution Respirometry

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

Development of a 68Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression

Spontaneous Murine Model of Anaplastic Thyroid Cancer

An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue

Synthesis of Single-Crystalline Core-Shell Metal-Organic Frameworks

A Scalable Balz-Schiemann Reaction Protocol in a Continuous Flow Reactor

Versatile Technique to Produce a Hierarchical Design in Nanoporous Gold

3-D Time-Lapse Imaging of Cell Wall Dynamics Using Calcofluor in the Moss Physcomitrium patens

In Vitro Investigation of the Effects of the Hyaluronan-Rich Extracellular Matrix on Neural Crest Cell Migration

Fabrication of a Low-Cost, Fiber-Coupled, and Air-Spaced Fabry-Pérot Etalon

Elucidating the Metabolism of 2,4-Dibromophenol in Plants

Screening Sperm for the Rapid Isolation of Germline Edits in Zebrafish

Development of a ⁶⁸Gallium-Labeled D-Peptide PET Tracer for Imaging Programmed Death-Ligand 1 Expression