JoVE Table of Contents: Issue 179, January 2022

An Open-Source, Fully Customizable 5-Choice Serial Reaction Time Task Toolbox for Automated Behavioral Training of Rodents

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Journal (Behavior)

Moxibustion Strategies for Mice

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Journal (Behavior)

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

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Journal (Biochemistry)

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Methods Collections

Transverse Sectioning of Mature Rice (<em>Oryza sativa</em> L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

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Journal (Biochemistry)

Ready-To-Use qPCR for Detection of DNA from <em>Trypanosoma cruzi</em> or Other Pathogenic Organisms

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

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Journal (Biochemistry)

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Methods Collections

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

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Journal (Biochemistry)

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Methods Collections

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

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Journal (Biochemistry)

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Methods Collections

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

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Journal (Biochemistry)

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Methods Collections

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

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Journal (Biochemistry)

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Methods Collections

In Vitro Preparation of Actively Maturing Bovine Articular Cartilage Explants for X-Ray Phase Contrast Imaging

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Journal (Bioengineering)

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

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Journal (Bioengineering)

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Methods Collections

Engineered Lung Tissues Prepared from Decellularized Lung Slices

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Journal (Bioengineering)

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Methods Collections

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

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Journal (Bioengineering)

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Methods Collections

Optical Coherence Tomography Based Biomechanical Fluid-Structure Interaction Analysis of Coronary Atherosclerosis Progression

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Journal (Bioengineering)

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Methods Collections

The Muscle Cuff Regenerative Peripheral Nerve Interface for the Amplification of Intact Peripheral Nerve Signals

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Journal (Bioengineering)

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

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Journal (Bioengineering)

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Methods Collections

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

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Journal (Bioengineering)

Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

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Journal (Bioengineering)

Cantilever Bending of Murine Femoral Necks

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Journal (Bioengineering)

Culturing and Screening the Plant Parasitic Nematode <em>Ditylenchus dipsaci</em>

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

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Journal (Biology)

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Methods Collections

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity <em>In Vitro</em>

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

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Journal (Biology)

Studying Membrane Protein Trafficking in <em>Drosophila</em> Photoreceptor Cells Using eGFP-Tagged Proteins

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

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Journal (Biology)

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Methods Collections

Recording Gap Junction Current from <em>Xenopus</em> Oocytes

Recording Gap Junction Current from Xenopus Oocytes

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Journal (Biology)

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

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Journal (Biology)

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Methods Collections

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

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Journal (Biology)

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Methods Collections

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies

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Journal (Biology)

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Methods Collections

Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System

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Journal (Biology)

Single-Particle Interferometric Reflectance Imaging Characterization of Individual Extracellular Vesicles and Population Dynamics

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Journal (Biology)

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Methods Collections

Organ Culture System for Assessing the Toxicity of Intraocular Treatment Excipients and Pharmaceuticals

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Journal (Biology)

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

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Journal (Biology)

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Methods Collections

Optical Clearing of Plant Tissues for Fluorescence Imaging

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Journal (Biology)

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Methods Collections

Longitudinal Intravital Imaging Through Clear Silicone Windows

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

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Journal (Chemistry)

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Methods Collections

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

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Journal (Developmental Biology)

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Methods Collections

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

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Journal (Developmental Biology)

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Methods Collections

Assembly and Characterization of an External Driver for the Generation of Sub-Kilohertz Oscillatory Flow in Microchannels

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Journal (Engineering)

Adsorptive Bioprocess Improves Yield of Melanin from <em>Pseudomonas stutzeri</em>

Adsorptive Bioprocess Improves Yield of Melanin from Pseudomonas stutzeri

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Journal (Engineering)

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Methods Collections

Evaluating the Electrochemical Properties of Supercapacitors using the Three-Electrode System

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Journal (Engineering)

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Methods Collections

Rapid Testing of Resistance of Timber to Biodegradation by Marine Wood-Boring Crustaceans

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Journal (Environment)

Leveraging Micro-CT Scanning to Analyze Parasitic Plant-Host Interactions

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Journal (Environment)

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Methods Collections

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

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Journal (Environment)

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Methods Collections

Rapid Isolation of Wild Nematodes by Baermann Funnel

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Journal (Genetics)

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Methods Collections

Chemical Cartography Approaches to Study Trypanosomatid Infection

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Journal (Immunology and Infection)

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Methods Collections

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

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Journal (Immunology and Infection)

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Methods Collections

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

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Journal (Medicine)

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Methods Collections

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

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Journal (Medicine)

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Methods Collections

Surgical Techniques to Optimize Ovarian Reserve during Laparoscopic Cystectomy for Ovarian Endometrioma

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Journal (Medicine)

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Methods Collections

Four-Dimensional Computed Tomography-Guided Valve Sizing for Transcatheter Pulmonary Valve Replacement

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Journal (Medicine)

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Methods Collections

Endoscopic Cholesteatoma Surgery

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Journal (Medicine)

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Methods Collections

High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

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Journal (Medicine)

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Methods Collections

Induction of Myocardial Infarction and Myocardial Ischemia-Reperfusion Injury in Mice

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Journal (Medicine)

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Methods Collections

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

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Journal (Medicine)

Intracerebral Transplantation and <em>In Vivo</em> Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

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Journal (Neuroscience)

Modified Spared Nerve Injury Surgery Model of Neuropathic Pain in Mice

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Journal (Neuroscience)

Imaging Mitochondrial Ca<sup>2+</sup> Uptake in Astrocytes and Neurons using Genetically Encoded Ca<sup>2+</sup> Indicators (GECIs)

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)

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Journal (Neuroscience)

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Methods Collections

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

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Journal (Neuroscience)

Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord <em>via</em> the Cisterna Magna in Mice

Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice

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Journal (Neuroscience)

A Flexible Platform for Monitoring Cerebellum-Dependent Sensory Associative Learning

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Journal (Neuroscience)

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Methods Collections

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

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Journal (Neuroscience)

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Methods Collections

Inhoudsopgave

Archiveren: Issue 179, January 2022

GA NAAR

An Open-Source, Fully Customizable 5-Choice Serial Reaction Time Task Toolbox for Automated Behavioral Training of Rodents

Moxibustion Strategies for Mice

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Transverse Sectioning of Mature Rice (Oryza sativa L.) Kernels for Scanning Electron Microscopy Imaging Using Pipette Tips as Immobilization Support

Ready-To-Use qPCR for Detection of DNA from Trypanosoma cruzi or Other Pathogenic Organisms

Cellular Membrane Affinity Chromatography Columns to Identify Specialized Plant Metabolites Interacting with Immobilized Tropomyosin Kinase Receptor B

Synthesis of Masarimycin, a Small Molecule Inhibitor of Gram-Positive Bacterial Growth

Measurement of Protein Import Capacity of Skeletal Muscle Mitochondria

Quantifying Bacterial Surface Swarming Motility on Inducer Gradient Plates

In Vitro Preparation of Actively Maturing Bovine Articular Cartilage Explants for X-Ray Phase Contrast Imaging

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Engineered Lung Tissues Prepared from Decellularized Lung Slices

Experimental and Data Analysis Workflow for Soft Matter Nanoindentation

Optical Coherence Tomography Based Biomechanical Fluid-Structure Interaction Analysis of Coronary Atherosclerosis Progression

The Muscle Cuff Regenerative Peripheral Nerve Interface for the Amplification of Intact Peripheral Nerve Signals

Microtubule Plus-End Dynamics Visualization in Huntington's Disease Model based on Human Primary Skin Fibroblasts

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood

Cantilever Bending of Murine Femoral Necks

Culturing and Screening the Plant Parasitic Nematode Ditylenchus dipsaci

Human Liver Microphysiological System for Assessing Drug-Induced Liver Toxicity In Vitro

Studying Membrane Protein Trafficking in Drosophila Photoreceptor Cells Using eGFP-Tagged Proteins

Recording Gap Junction Current from Xenopus Oocytes

Bioinformatics Resources for the Study of Glycan-Mediated Protein Interactions

Longitudinal Intravital Microscopy Using a Mammary Imaging Window with Replaceable Lid

Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit Flies

Process Development for the Production and Purification of Adeno-Associated Virus (AAV)2 Vector using Baculovirus-Insect Cell Culture System

Single-Particle Interferometric Reflectance Imaging Characterization of Individual Extracellular Vesicles and Population Dynamics

Organ Culture System for Assessing the Toxicity of Intraocular Treatment Excipients and Pharmaceuticals

Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples

Optical Clearing of Plant Tissues for Fluorescence Imaging

Longitudinal Intravital Imaging Through Clear Silicone Windows

Using a Cyclic Ion Mobility Spectrometer for Tandem Ion Mobility Experiments

Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell Differentiation

Assembly and Characterization of an External Driver for the Generation of Sub-Kilohertz Oscillatory Flow in Microchannels

Adsorptive Bioprocess Improves Yield of Melanin from Pseudomonas stutzeri

Evaluating the Electrochemical Properties of Supercapacitors using the Three-Electrode System

Rapid Testing of Resistance of Timber to Biodegradation by Marine Wood-Boring Crustaceans

Leveraging Micro-CT Scanning to Analyze Parasitic Plant-Host Interactions

Quantification of the Potential Impact of Glyphosate-Based Products on Microbiomes

Rapid Isolation of Wild Nematodes by Baermann Funnel

Chemical Cartography Approaches to Study Trypanosomatid Infection

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Standardization and Maintenance of 3D Canine Hepatic and Intestinal Organoid Cultures for Use in Biomedical Research

Measurement of Tissue Non-Heme Iron Content using a Bathophenanthroline-Based Colorimetric Assay

Surgical Techniques to Optimize Ovarian Reserve during Laparoscopic Cystectomy for Ovarian Endometrioma

Four-Dimensional Computed Tomography-Guided Valve Sizing for Transcatheter Pulmonary Valve Replacement

Endoscopic Cholesteatoma Surgery

High-speed Video Microscopy Analysis for First-line Diagnosis of Primary Ciliary Dyskinesia

Induction of Myocardial Infarction and Myocardial Ischemia-Reperfusion Injury in Mice

Application of Optical Coherence Tomography to a Mouse Model of Retinopathy

Intracerebral Transplantation and In Vivo Bioluminescence Tracking of Human Neural Progenitor Cells in the Mouse Brain

Modified Spared Nerve Injury Surgery Model of Neuropathic Pain in Mice

Imaging Mitochondrial Ca2+ Uptake in Astrocytes and Neurons using Genetically Encoded Ca2+ Indicators (GECIs)

A Gravity-Fed Transcardial Perfusion Method for Histologic Analysis of the Mouse Central Nervous System

Stereotaxic Surgical Approach to Microinject the Caudal Brainstem and Upper Cervical Spinal Cord via the Cisterna Magna in Mice

A Flexible Platform for Monitoring Cerebellum-Dependent Sensory Associative Learning

Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain

Imaging Mitochondrial Ca²⁺ Uptake in Astrocytes and Neurons using Genetically Encoded Ca²⁺ Indicators (GECIs)