Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer InterferometryTripthi Battapadi 1, Madhumita Sridharan 1, Degang Liu 2, John Turchi 3, Lata Balakrishnan 1
1Department of Biology, Indiana University Indianapolis, 2Sartorius Corporation, 3Department of Medicine, Indiana University School of Medicine
This article describes a protocol for studying DNA-protein interactions using a streptavidin-based biolayer interferometry (BLI) system. It outlines the essential steps and considerations for utilizing either basic or advanced binding kinetics to determine the equilibrium binding affinity (KD) of the interaction.
