Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

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3.8K Views

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05:46 min

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June 9th, 2020

DOI :

10.3791/61371-v

June 9th, 2020

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Daria Barwinska¹, Michael J. Ferkowicz¹, Ying-Hua Cheng¹, Seth Winfree¹^,², Kenneth W. Dunn¹, Katherine J. Kelly¹, Timothy A. Sutton¹, Brad H. Rovin³, Samir V. Parikh³, Carrie L. Phillips⁴, Pierre C. Dagher¹, Tarek M. El-Achkar¹, Michael T. Eadon¹, For The Kidney Precision Medicine Project

¹Department of Medicine, Indiana University School of Medicine, ²Department of Cellular & Integrative Physiology, Indiana University School of Medicine, ³Division of Nephrology, Department of Medicine, Ohio State University Wexner Medical Center, ⁴Division of Pathology, Indiana University School of Medicine

Podsumowanie

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Laser Microdissection

Transcriptomic Analysis

Kidney Tissue

Spatial Transcriptomics

Cryostat

RNA Sequencing

Tissue Economy

Lowly Expressed Transcripts

Microcentrifuge Collection Tubes

PCR Work

Laser Power

Rozdziały w tym wideo

0:05

Introduction

0:41

Cryosectioning and Laser Microdissection (LMD)

3:39

Results: Representative Human Kidney Regional Transcriptomics Analyses

5:05

Conclusion

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