Patch Angioplasty in the Rat Aorta or Inferior Vena Cava

Podsumowanie

We have established a model of pericardial patch angioplasty that can be used in either small-diameter veins or arteries. This model can be used to compare venous and arterial neointimal hyperplasia formation.

Streszczenie

Pericardial patches are commonly used in vascular surgery to close vessels. To facilitate studies of the neointimal hyperplasia that forms on the patch, we developed a rat model of patch angioplasty that can be used in either a vein or an artery, creating a patch venoplasty or a patch arterioplasty, respectively. Technical aspects of this model are discussed. The infra-renal IVC or aorta are dissected and then clamped proximally and distally. A 3 mm venotomy or arteriotomy is performed in the infrarenal inferior vena cava or aorta of 6 to 8 week-old Wistar rats. A bovine pericardial patch (3 mm x 1.5 mm x 0.6 mm) is then used to close the site using a 10-0 nylon suture. Compared to arterial patches, venous patches show increased neointimal thickness on postoperative day 7. This novel model of pericardial patch angioplasty can be used to examine neointimal hyperplasia on vascular biomaterials, as well as to compare the differences between the arterial and venous environments.

Wprowadzenie

Vascular surgeons commonly close blood vessels with pericardial patches, either bovine or porcine, to reduce the risk of restenosis^1,2. In arterial surgery, pericardial patch angioplasty reduces the rate of restenosis compared to a primary closure^3,4. Patch venoplasty is also similarly performed to close veins, although less frequently when compared to arterial procedures^5,6. Patches typically develop neointimal hyperplasia on their luminal surfaces; however, the mechanism of neointimal formation remains unclear, especially in the comparison of differences in neointimal formation between the arterial and venous environments. We previously showed that pericardial patches attract progenitor cells in both the arterial and the venous environments and that venous patch angioplasty shows more aggressive neointimal formation as compared to the arterial patches^7,8.

In this study, we describe the rat model of pericardial patch angioplasty that is common to closing both veins and arteries and that may be used to study neointimal formation; this method is a small-animal model of an operative technique commonly used in human patients. The rat model has a larger diameter than the mouse model, facilitating technical aspects of the surgery and improving the learning curve for the model.

Protokół

Approval by the appropriate Institutional Animal Care and Use Committee must be obtained, indicating that your protocol follows the guidelines of your institution's animal research ethics committee.

1. Anesthesia and Pre-operative Procedures

1. Anesthetize male Wistar rats, weighing around 200 g, with vaporized 3% isoflurane and 1 L/min oxygen administered into an acrylic induction chamber.
2. Confirm adequate anesthesia through a lack of reaction to a toe and tail pinch. Place ointment on the eyes to prevent dryness while the animals are under anesthesia. Position the each rat supine on the plastic operation plate and position a silicone mask to deliver vaporized 2 - 3% isoflurane by continuous inhalation. Do not leave an animal unattended until it has regained sufficient consciousness to maintain sternal recumbency.
3. While wearing sterile gloves, remove the ventral abdomen hair using a hair remover. Buprenorphine, either subcutaneously or intraperitoneally, is frequently given at this point.

2. Operative Procedures

1. Patch venoplasty
   1. Before the surgery, trim the bovine pericardial patch using a microscissor to a 3 mm x 1.5 mm x 0.6 mm size. Soak the patch in 10 mL of sterilized saline for 5 min in a cell culture dish.
   2. Prepare the incision site with a topical antiseptic according to your institutional protocol and apply a surgical drape. Use sterile gloves and instruments to maintain aseptic technique throughout the surgery.  Local anesthesia can be injected into the site of the planned skin incision.
   3. Using a scalpel, make a midline abdominal incision extending from the level of the xiphoid to just above the pubis.
   4. Insert a retractor and eviscerate all bowels from the abdominal cavity towards the right side. Wrap the bowels in gauze soaked with saline.
   5. Expose the infrarenal vena cava (IVC). The site of patch implantation is approximately 2 mm below the level of the origin of the renal veins; dissect the IVC free at this site. Ligate and divide all lumbar and tributary veins at this level using 6-0 nylon sutures.
   6. Place two microsurgery clips across both the proximal and distal infarenal IVC. Perform a venotomy with a 25 G needle by puncturing the anterior wall of the IVC, and then extend it to around 3 mm using scissors (Figure 1A).
   7. Close the venotomy with the patch using running or interrupted 10-0 nylon sutures (Figure 1B).
   8. Complete the venoplasty closure. Remove the distal clamp first to vent the patch and then remove the proximal clamp to restore blood flow to the IVC.
   9. Confirm hemostasis by observing for 30 s without compression. Return the bowels to their natural position. Close the abdomen with a running suture according to approved animal protocols.
2. Patch arterioplasty
   1. Before the surgery, trim the bovine pericardial patch using a microscissor to a 3 mm x 1.5 mm x 0.6 mm size. Soak it in sterilized saline.
   2. Prepare the incision site with a topical antiseptic and apply a surgical drape. Use sterile gloves and instruments to maintain aseptic technique throughout the surgery.
   3. Using a scalpel, make a midline abdominal incision extending from the level of the xiphoid to just above the pubis.
   4. Insert a retractor and eviscerate all bowels from the abdominal cavity towards the right side. Wrap the bowels in gauze soaked with saline.
   5. Expose the aorta. The site of patch implantation is approximately 2 mm below the level of the origin of the renal arteries; dissect the aorta free at this site. Ligate and divide all lumbar arteries at this level using 6-0 nylon sutures.
   6. Clamp the infrarenal aorta. Use a 25 G needle to perform an arteriotomy by puncturing the anterior wall of the aorta, and then extend it to around 3 mm using scissors (Figure 1C).
   7. Close the arteriotomy with the patch using running 10-0 nylon sutures (Figure 1D). Complete the arterioplasty closure. Remove the distal clamp first to vent the patch and then remove the proximal clamp to restore blood flow to the aorta.
   8. Confirm hemostasis by observing for 30 s without compression. Return the bowels to their natural position. Close the abdomen with a running suture according to approved animal protocols.

3. Post-operative Procedures

1. After the closure of the abdomen, discontinue the anesthesia. Apply post-operative care, including analgesia and wound care, in accordance with instructions recommended by the Institutional Animal Care and Use Committee.
2. For analgesia, use buprenorphine at 0.1 mg/kg intramuscularly no less than every 12 h for 24 h following the surgical procedures. Check the status of the animal every day in the animal room, ensuring proper recovery from the peri-operative period as well as adequate treatment of post-surgical pain. Do not return an animal that has undergone surgery to the company of other animals until it has fully recovered.

Wyniki

After the patch angioplasty, there was no thrombosis in either the venous (n = 42) or arterial (n = 24) patch groups. Survival at day 7 was 100% in both groups with either a venous or arterial patch.

The patches were harvested on day 7 and stained with hematoxylin and eosin, and the neointimal thickness was compared as previously described⁷. Because of the distinct morphological difference between the patch and the ne...

Dyskusje

We describe a patch angioplasty model in the rat that can be used in either veins or arteries, creating a patch venoplasty or patch arterioplasty, respectively. Using this pericardial patch model, we showed aggressive and stable neointimal hyperplasia in the rat IVC venoplasty compared to the aortic arterioplasty (Figure 2)^7,8.

Several technical aspects are notable. In the aortic arterioplasty, it is important that the...

Materiały

Name	Company	Catalog Number	Comments
Bovine pericardial patch	LeMaitre Vascular
Vascular clamp	Roboz Surgical Instrument Co.	RS-5424
Clamp applying forceps	Roboz Surgical Instrument Co	RS-5410
10-0 Prolene suture	Arosurgical Co.	T05A10N10-13