A Direct, Regioselective and Atom-Economical Synthesis of 3-Aroyl-N-hydroxy-5-nitroindoles by Cycloaddition of 4-Nitronitrosobenzene with Alkynones

Podsumowanie

3-Aroyl-N-hydroxy-5-nitroindoles were synthesized by cycloaddition of 4-nitronitrosobenzene with a conjugated terminal alkynone in a one-step thermal procedure. Preparation of the nitrosoarene and of the alkynones were adequately reported and respectively through oxidation procedures on the corresponding aniline and on the alkynol.

Streszczenie

We introduced a regioselective and atom-economical procedure for the synthesis of 3-substituted indoles by annulation of nitrosoarenes with ethynyl ketones. The reactions were carried out achieving indoles without any catalyst and with excellent regioselectivity. No traces of 2-aroylindole products were detected. Working with 4-nitronitrosobenzene as starting material, the 3-aroyl-N-hydroxy-5-nitroindole products precipitated from the reaction mixtures and were isolated by filtration without any further purification technique. Differently from the corresponding N-hydroxy-3-aryl indoles that, spontaneously in solution, give dehydrodimerization products, the N-hydroxy-3-aroyl indoles are stable and no dimerization compounds were observed.

Wprowadzenie

Aromatic C-nitroso compounds¹ and alkynones² are versatile reactants that are continuously and deeply used and studied as starting materials for the preparation of high valuable compounds. Nitrosoarenes play an ever-growing role in the organic synthesis. They are used for many different purposes (e.g., hetero Diels-Alder reaction^3,4, Nitroso-Aldol reaction^5,6, Nitroso-Ene reaction⁷, synthesis of azocompounds^8,9,10). Very recently they were even used as starting materials to afford different heterocyclic compounds^11,12,13. In the last decades, conjugated ynones were investigated for their role as very interesting and useful scaffolds in the achievement of many high valuable derivatives and heterocyclic products^{14,15,16,17,18}. C-Nitrosoaromatics can be afforded by oxidation reactions of the corresponding and commercially available anilines using different oxidizing agents as potassium peroxymonosulfate (KHSO₅·0.5KHSO₄·0.5K₂SO₄)¹⁹, Na₂WO₄/H₂O₂²⁰, Mo(VI)-complexes/H₂O₂^21,22,23, selenium derivatives²⁴. Alkynones are easily prepared by the oxidation of the corresponding alkynols using various oxidants (CrO₃²⁵ even known as Jones' reagent or mild reactants as MnO₂²⁶ and Dess-Martin periodinane²⁷). The alkynols can be achieved by direct reaction of ethynylmagnesium bromide with commercially available arylaldehydes or heteroarylaldehydes²⁸.

Indole is probably the most studied heterocyclic compound and indole derivatives have wide and various applications in many different research fields. Both medicinal chemists and material scientists produced many indole-based products that cover different functions and potential activities. Indole compounds have been investigated by many research groups and both naturally occurring products and synthetic derivatives containing the indole framework show relevant and peculiar properties^29,30,31,32. Among the plethora of indole compounds, the 3-aroylindoles have a relevant role among molecules that show biological activities (Figure 1). Different indole products belong to diverse classes of pharmaceutical candidates to become potential novel drugs³³. Synthetic and naturally occurring 3-aroylindoles are known to play a role as antibacterial, antimitotic, analgesic, antiviral, anti-inflammatory, antinociceptic, antidiabetic and anticancer^34,35. The '1-hydroxyindole hypothesis' was provocatively introduced by Somei and coworkers as an interesting and stimulating supposition to support the biological role of N-hydroxyindoles in the biosynthesis and functionalization of indole alkaloids^36,37,38,39. This assumption was recently reinforced by the observation of many endogen N-hydroxy heterocyclic compounds that show relevant biological activities and an interesting role for many purposes as pro-drugs⁴⁰. In the recent years, the search for novel active pharmaceutical ingredients revealed that different N-hydroxyindole fragments were detected and discovered in natural products and bioactive compounds (Figure 2): Stephacidin B⁴¹ and Coproverdine⁴² are known as antitumor alkaloids, Thiazomycins⁴³ (A and D), Notoamide G⁴⁴ and Nocathacins^45,46,47 (I, III, and IV) are deeply studied antibiotics, Opacaline B⁴⁸ is a natural alkaloid from ascidian Pseudodistoma opacum and Birnbaumin A and B are two pigments from Leucocoprinus birnbaumii⁴⁹. New and efficient N-hydroxyindole-based inhibitors of LDH-A (Lactate DeHydrogenase-A) and their ability to reduce the glucose to lactate conversion inside the cell were developed^{50,51,52,53,54,55,56}. Other researchers repeated that indole compounds, that did not show biological activities, became useful pro-drugs after the insertion of a N-hydroxy group⁵⁷.

A motif of debate was the stability of N-hydroxyindoles and some of these compounds gave easily a dehydrodimerization reaction that leads to the formation of a class of novel compounds, subsequently renamed as kabutanes^58,59,60,61, by the formation of a new C-C bond and two new C-O bonds. Due to the importance of stable N-hydroxyindoles the study of different synthetic approaches for the easy preparation of such compounds becomes a fundamental topic. In a previous research by some of us, an intramolecular cyclization by a Cadogan-Sundberg-type reaction was reported using nitrostyrenes and nitrostilbene as starting materials⁶². In the last decades we developed a novel cycloaddition between nitro- and nitrosoarenes with different alkynes in an intermolecular fashion affording indoles, N-hydroxy- and N-alkoxyindoles as major products (Figure 3).

At the beginning, using aromatic and aliphatic alkynes^{63,64,65,66,67} the reactions were carried out in large excess of alkyne (10 or 12-fold) and sometimes under alkylative conditions to avoid the formation of kabutanes. 3-Substituted indole products were achieved regioselectively in moderate to good yields. Using electron poor alkynes, like 4-ethynylpyrimidine derivatives as privileged substrates we could carry out the reactions for this one-pot synthetic protocol using a 1/1 nitrosoarene/alkyne molar ratio⁶⁸. With this protocol, an interesting class of kinase inhibitors as meridianins, marine alkaloids isolated from Aplidium meridianum⁶⁹, was prepared showing a different approach to meridianins through an indolization procedure (Figure 4)⁶⁸. Meridianins were generally produced so far with synthetic tools starting from preformed indole reactants. To the best of our knowledge, only a couple of methodologies reported the total synthesis of meridianins or meridianin derivatives through an indolization procedure^68,70.

In a more recent development on the use of electron poor alkynes it was worthwhile to test the employ of terminal alkynones as substrates for the indolization procedure and this led us to disclose an intermolecular synthetic technique to afford 3-aroyl-N-hydroxyindole products^71,72. Analogously to the process studied for the preparation of meridianins, using terminal arylalkynone compounds the 1/1 Ar-N=O/Ar-(C=O)-C≡CH molar ratio was used (Figure 5). Working with alkynones as privileged starting materials, the general indole synthesis was performed with different reactants exploring a wide substrate survey and changing the nature of the substituents both on nitrosoarenes and on the aromatic ynones. Electron-withdrawing groups on the C-nitrosaromatic compound led us to observe an improvement both in reaction times and in products yields. An interesting synthetic approach that makes easily available a stable library of these compounds could be very useful and, after a preliminary study, we optimized our synthetic protocol using this stoichiometric reaction between alkynones and 4-nitronitrosobenzene to afford stable 3-aroyl-N-hydroxy-5-nitroindoles. Basically, this easy access to N-hydroxyindoles led us to evidence as the cycloaddition reaction between nitrosoarene and alkynone is a very atom-economical process.

Protokół

1. Preliminary preparation of the Jones Reagent

1. Add 25 g (0.25 mol) of chromium trioxide using a spatula in a 500 mL beaker that contains a magnetic stirring bar.
2. Add 75 mL of water and keep the solution under magnetic stirring.
3. Add slowly 25 mL of concentrated sulfuric acid with careful stirring and cooling in an ice-water bath.
   NOTE: Now the solution is ready and is stable and usable for many oxidation procedures; the concentration of the solution prepared by this procedure is 2.5 M.

2. Synthesis of 1-phenyl-2-propyne-1-one

1. Add 75 mL of acetone in an open-air round bottom flask that contains a magnetic stirring bar.
2. Add 2.0 g (15.13 mmol) of 1-phenyl-2-propyne-1-ol via a glass Pasteur pipette.
3. Keep the reaction mixture at 0 °C and under magnetic stirring.
4. Add a solution of Jones reagent dropwise till the presence of a persistent orange color.
5. Add 2-propanol dropwise till the excess of Cr(VI) reactant is consumed to the point of a green color.
6. Filter the solution through a pad of diatomaceous earth.
7. Concentrate the washings by rotary evaporation obtaining an oil.
8. Dissolve the oil in 100 mL of CH₂Cl₂ and put in a separatory funnel.
9. Wash this organic phase with a saturated solution of NaHCO₃ (2 x 125 mL).
10. Wash the organic layer with brine (125 mL).
11. Dry the organic solution over anhydrous Na₂SO₄ and filter it.
12. Evaporate the solution obtaining 1.77 g of 1-phenyl-2-propyne-1-one as a yellow solid (quantitative yield).
13. Leave the solid to dry in vacuum.
14. Analyze and characterize by ¹H-NMR.

3. Preparation of 4-nitronitrosobenzene

1. Add 16 g of potassium peroxymonosulfate (2KHSO₅·KHSO₄·K₂SO₄) (26 mmol) using a spatula in a beaker, open to air that contains a magnetic stirring bar.
2. Add 150 mL of water and keep the solution at 0 °C under magnetic stirring.
3. Add 3.6 g of 4-nitroaniline (26 mol) using a spatula.
4. Stir the suspension at room temperature.
5. Check the reaction by TLC till the complete conversion of 4-nitroaniline (Rf_{4-Nitroaniline} = 0.44, Rf_{4-Nitronitrosobenzene} = 0.77; CH₂Cl₂ as eluent).
6. Filter the crude reaction mixture on a Buchner after 48 h.
7. Put the solid in a one-neck round bottom flask.
8. Recrystallize the solid in methanol (50 mL).
9. Warm the suspension using a heat gun till boiling point of methanol and filter immediately the hot suspension.
10. Discard the solid and reuse it eventually for another recrystallization.
11. Filter the second precipitate formed in the Erlenmeyer flask when the solution reaches room temperature.
12. Leave the solid to dry in vacuum on a Buchner funnel.
13. Characterize the solid by ¹H-NMR.

4. Synthesis of 3-benzoyl-1-hydroxy-5-nitroindole

1. Connect all the oven dried glassware (a 250 mL two neck round bottom flask containing a magnetic stirring bar, a stopcock, a refrigerant and a joint to connect to vacuum/nitrogen system) and put under vacuum for 30 min.
2. At room temperature, after some cycles of vacuum/nitrogen, flush all the glassware with nitrogen and leave it under inert atmosphere.
3. Add 1.52 g (10 mmol) of 4-nitronitrosobenzene under inert atmosphere.
4. Add 1.30 g (10 mmol) of 1-phenyl-2-propyne-1-one.
5. Add 80 mL of toluene via a syringe and keep the reaction mixture under magnetic stirring at 80 °C.
6. After few minutes, check the complete solubilization of the reactants.
7. Verify the formation of an orange precipitate after about 30-40 min at 80 °C.
8. After the complete precipitation of an orange solid (about 2.5 h), turn off the heating and leave the reaction to reach room temperature.
9. Filter the mixture and collect 3-benzoyl-1-hydroxy-5-nitroindole as an orange solid on a Buchner funnel.
10. Keep under vacuum to dryness.
11. Analyze and characterize the solid product by ¹H- and ¹³C-NMR, FT-IR, and HRMS.

Wyniki

The preparation of 4-nitronitrosobenzene 2 was achieved by oxidation of 4-nitroaniline 1 by reaction with potassium peroxymonosulfate as reported in Figure 6. The product 2 was obtained in 64% yield after recrystallization in MeOH (twice) with 3-5% contamination of 4,4'-bis-nitro-azoxybenzene 6. The structure of product 2 was confirmed by ¹H-NMR (Figure 7). ^1...

Dyskusje

The reaction for the indole synthesis between nitrosoarenes and alkynones shows a very high versatility and a strong and wide application. In a previous report, we could generalize our synthetic protocol working with different C-nitrosoaromatics and substituted terminal arylalkynones or heteroarylalkyones⁷². The procedure shows a deep substrate survey and a high functional group tolerance and both electron-withdrawing groups and electron-donor groups were present both in nitrosoarene and ...

Materiały

Name	Company	Catalog Number	Comments
4-Nitroaniline	TCI Chemicals	N0119
Acetone	TCI Chemicals	A0054
1-Phenyl-2-propyne-1-ol	TCI Chemicals	P0220
Celite 535	Fluorochem	44931
Dichloromethane	TCI Chemicals	D3478
Sodium hydrogen carbonate	Sigma Aldrich	S5761
Sodium chloride	Sigma Aldrich	746398
Sodium sulfate anhydrous	Sigma Aldrich	239313
Oxone	TCI Chemicals	O0310
Methanol	TCI Chemicals	M0628
Toluene	TCI Chemicals	T0260
Chromium Trioxide	Sigma Aldrich	236470
Dichloromethane anhydrous	TCI Chemicals	D3478
Hexane anhydrous	TCI Chemicals	H1197