To begin, gently position the mouse with its belly up and the head slightly down. Grasp the back skin to secure the abdominal area and insert a one milliliter syringe needle, one centimeter to the right of the midline of the abdomen at the intersection of the line, connecting both thighs. Gradually insert the one milliliter syringe needle to a depth of three to five millimeters under the skin.
Insert the needle approximately 0.3 to 0.5 millimeters into the abdominal cavity at a 45 degree angle. Once the needle tip passes through the abdominal muscle, notice a sudden decrease in resistance. Slowly inject 0.1 milliliters of the azoxymethane injection solution per 10 grams of body weight into the mice.
Subsequently, retract the syringe gently. Then add 500 milliliters of sterile distilled water to 10 grams of DSS. To prepare a 2%DSS solution.
Mix the solution with a vortex mixer and store at four degrees Celsius until use, administer the prepared LJZD and five amino salicylic acid solution daily at a consistent time via gastric gavage to the respective groups at week seven and week 15.
