To begin, obtain previously prepared stained lung cancer tissue sample slides. For automatic scanning of the stained slides, manually set the exposure time and scanning program for each fluorescence channel. Drag the multicolor fluorescence image file obtained from the original scan into the software and set the image type to fluorescence.
Click the brightness and contrast button and check the channels on the panel. Click outside and then on the channel to select the fluorescence channel of interest. Drag the minimum display and maximum display to adjust the brightness and contrast of each channel.
Once the view to be saved is determined, click on show slide overview and show cursor location to remove these two contents and keep the scale bar. Click file followed by export snapshot and current viewer content To export a PNG file. The non-small cell lung squamous carcinoma tissues showed that macrophages and CD eight positive T-cells significantly infiltrated around and within carcinoma foci.
HMGCS1 protein exhibited widespread expression in squamous carcinoma cell plasma.
