Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

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15.1K Views

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09:26 min

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November 17th, 2011

DOI :

10.3791/3399-v

November 17th, 2011

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Stacy L. Gelhaus¹^,², A. Clementina Mesaros¹^,², Ian A. Blair¹^,²

¹Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania , ²Department of Pharmacology, University of Pennsylvania

Podsumowanie

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Cellular Lipid Extraction

Targeted Stable Isotope Dilution

Liquid Chromatography mass Spectrometry Analysis

Fatty Acids

Arachidonic Acid

Linoleic Acid

Oxidized Bioactive Lipids

Stereoisomers

Enzymatic Metabolism

Reactive Oxygen Species

Liquid liquid Extraction

Stable Isotope Internal Standards

Ion Suppression

Mass Spectrometry Analysis

Rozdziały w tym wideo

0:05

Title

1:26

Collection of Media and Cell Samples

2:00

Extraction of Lipids from Media

3:02

Extraction of Lipids from Cell Lysate

5:16

Pentafluorylbenzylbromide (PFB) Derivatization

5:40

SID-LC-ECAPCI-MS Analysis

7:46

Targeted Lipidomics Profile

9:03

Conclusion

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