Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

16.1K Views

09:26 min

November 17th, 2011

DOI :10.3791/3399-v

November 17th, 2011

Transcript

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Cellular Lipid Extraction

Chapters in this video

0:05

Title

1:26

Collection of Media and Cell Samples

2:00

Extraction of Lipids from Media

3:02

Extraction of Lipids from Cell Lysate

5:16

Pentafluorylbenzylbromide (PFB) Derivatization

5:40

SID-LC-ECAPCI-MS Analysis

7:46

Targeted Lipidomics Profile

9:03

Conclusion

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