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In ovo Electroporation of miRNA-based Plasmids in the Developing Neural Tube and Assessment of Phenotypes by DiI Injection in Open-book Preparations

16.4K Views

12:41 min

October 16th, 2012

DOI :

10.3791/4384-v

October 16th, 2012


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Ovo Electroporation

Rozdziały w tym wideo

0:05

Title

1:15

Preparing Eggs for Electroporation

2:59

Electroporation of miRNA

4:49

Dissection of Embryos for Spinal Cord Preparations

7:28

Fixation of Open-books

8:11

DiI Injection into Commissural Neurons

9:48

Results: Promoter-driven Cell-type Specific Expression in Neural Tube Cross-section and Open-book Preparations

12:12

Conclusion

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