Methods Article

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

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17.8K Views

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09:59 min

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May 3rd, 2013

DOI :

10.3791/50047-v

May 3rd, 2013

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Chun A. Changou¹^,², Deanna L. Wolfson², Balpreet Singh Ahluwalia²^,³, Richard J. Bold⁴^,⁵, Hsing-Jien Kung⁵^,⁶, Frank Y.S. Chuang¹^,²^,⁵

¹Department of Biochemistry and Molecular Medicine, University of California, Davis , ²NSF Center for Biophotonics Science & Technology, University of California, Davis , ³University of Tromsø, ⁴Department of Surgery (Division of Surgical Oncology), University of California, Davis , ⁵UC Davis Comprehensive Cancer Center, University of California, Davis , ⁶Department of Biological Chemistry, University of California, Davis

Podsumowanie

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Quantitative Analysis

Autophagy

3D Fluorescence Microscopy

Widefield Deconvolution Microscopy

Structured Illumination Microscopy

Digital Image Analysis

Rozdziały w tym wideo

0:05

Title

1:27

Preparing Live Cell Specimens for Imaging

3:10

3D (Deconvolution) Fluorescence Microscopy

6:10

Results: Quantitative Image-Based Analysis of Cells Undergoing Autophagy

9:02

Conclusion

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