Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
17.8K Views
•
09:59 min
•
May 3rd, 2013
DOI :
May 3rd, 2013
•Chun A. Changou1,2, Deanna L. Wolfson2, Balpreet Singh Ahluwalia2,3, Richard J. Bold4,5, Hsing-Jien Kung5,6, Frank Y.S. Chuang1,2,5
1Department of Biochemistry and Molecular Medicine, University of California, Davis , 2NSF Center for Biophotonics Science & Technology, University of California, Davis , 3University of Tromsø, 4Department of Surgery (Division of Surgical Oncology), University of California, Davis , 5UC Davis Comprehensive Cancer Center, University of California, Davis , 6Department of Biological Chemistry, University of California, Davis
Chapters in this video
0:05
Title
1:27
Preparing Live Cell Specimens for Imaging
3:10
3D (Deconvolution) Fluorescence Microscopy
6:10
Results: Quantitative Image-Based Analysis of Cells Undergoing Autophagy
9:02
Conclusion
Related Videos
10:27
A Fluorescence Microscopy Assay for Monitoring Mitophagy in the Yeast Saccharomyces cerevisiae
25.4K Views
06:58
Evaluation of LC3-II Release via Extracellular Vesicles in Relation to the Accumulation of Intracellular LC3-positive Vesicles
529 Views
08:44
Quantitative Cell Biology of Neurodegeneration in Drosophila Through Unbiased Analysis of Fluorescently Tagged Proteins Using ImageJ
9.0K Views
09:00
Live Cell Imaging of Early Autophagy Events: Omegasomes and Beyond
18.4K Views
06:51
Analysis of Autophagy in Penicillium chrysogenum by Using Starvation Pads in Combination With Fluorescence Microscopy
8.5K Views
11:39
Assessing Autophagic Flux by Measuring LC3, p62, and LAMP1 Co-localization Using Multispectral Imaging Flow Cytometry
31.0K Views
14:08
Study of Protein-protein Interactions in Autophagy Research
9.0K Views
09:18
Measuring Diurnal Rhythms in Autophagic and Proteasomal Flux
4.6K Views
Flux-Based Assay for the Identification of Autophagy Modulators for Osteoarthritis
3.0K Views
08:07
Evaluating Autophagy Levels in Two Different Pancreatic Cell Models Using LC3 Immunofluorescence
1.4K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved