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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

14.3K Views

10:24 min

December 17th, 2012

DOI :

10.3791/50066-v

December 17th, 2012


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Keywords MRNA Localization

Rozdziały w tym wideo

0:05

Title

1:18

Preparation of Cells and Treatment with Translation Inhibitors

2:29

Digitonin Extraction and Cell Fixation

4:47

Fluorescent In situ Hybridization

6:21

Washing and Mounting the Stained Samples

8:08

Placental Alkaline Phosphatase and Cytochrome P450-8B1 mRNA ER-association

9:50

Conclusion

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